       Document 0545
 DOCN  M9640545
 TI    Endosomolytic activity of cationic liposomes enhances the delivery of
       human immunodeficiency virus-1 trans-activator protein (TAT) to
       mammalian cells.
 DT    9604
 AU    Huang L; Farhood H; Serbina N; Teepe AG; Barsoum J; Department of
       Pharmacology, University of Pittsburgh, School of; Medicine, PA 15261,
       USA.
 SO    Biochem Biophys Res Commun. 1995 Dec 26;217(3):761-8. Unique Identifier
       : AIDSLINE MED/96125309
 AB    We have explored the use of cationic liposomes to deliver the human
       immunodeficiency virus-1 trans-activator protein tat using a reporter
       gene expression assay. The human epidermoid carcinoma cell A431 stably
       transfected with a reporter gene under the control of human
       immunodeficiency virus-1 promoter was used as a target cell.
       Phosphatidylcholine-containing cationic liposomes had no detectable tat
       delivery activity. In contrast, delivery of tat was enhanced by up to
       150-fold using cationic liposomes enriched with dioleoyl
       phosphatidylethanolamine (DOPE), a lipid which readily transforms a
       bilayer into a nonbilayer structure. Enhanced delivery of tat by
       DOPE-containing liposomes was most likely the result of the
       endosomolytic activity of the liposome. This phospholipid-rich
       formulation showed no toxicity at concentrations sufficient for maximal
       delivery of tat. A variety of cationic liposome formulations which
       contain DOPE were tested successfully for tat delivery.
 DE    Cations  Drug Delivery Systems  Endocytosis  Endosomes/CHEMISTRY  Gene
       Products, tat/*ADMINISTRATION & DOSAGE  Human  HIV-1
       Liposomes/*CHEMISTRY  Support, U.S. Gov't, P.H.S.  Tumor Cells, Cultured
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

