       Document 0547
 DOCN  M9640547
 TI    Effect of leukocyte concentration and inoculum volume on the laboratory
       identification of cytomegalovirus in peripheral blood by the
       centrifugation culture-antigen detection methodology.
 DT    9604
 AU    Lipson SM; Falk LH; Lee SH; Jane and Dayton Brown and Dayton T. Brown,
       Jr., Virology; Laboratory, Department of Medicine, North Shore
       University; Hospital-Cornell University Medical College, Manhasset, NY
       11030,; USA.
 SO    Arch Pathol Lab Med. 1996 Jan;120(1):53-6. Unique Identifier : AIDSLINE
       MED/96136017
 AB    OBJECTIVE--To investigate the rate of Cytomegalovirus (CMV) detection in
       peripheral blood by shell vial assay-indirect immunofluorescent assay
       technology using two leukocyte inocula concentrations and different
       inoculum volumes containing equivalent cell concentrations.
       DESIGN--Leukocyte inocula concentrations of 2 x 10(5) and 4 x 10(5)
       cells per 0.2 mL were assayed for the presence of CMV by shell vial
       assay-indirect immunofluorescent assay. The effect of different inoculum
       volumes (0.2 and 0.4 mL) containing an equivalent cell concentration of
       4 x 10(5) was evaluated as well. The data were compared to conventional
       MRC-5 tube cultures, including blind passage. PATIENTS--Ninety-five
       patients (101 specimens) were tested sequentially. The test population
       consisted primarily of patients suffering from the acquired
       immunodeficiency syndrome. SETTING--The diagnostic virology laboratory,
       acquired immunodeficiency syndrome clinics, and hospital wards.
       RESULTS--Among the 101 specimens tested by shell vial assay-indirect
       immunofluorescent assay, the rate of CMV sensitivity increased by 36%
       using the higher leukocyte inoculum concentration of 4 x 10(5) cells per
       0.2 mL (P = .002; Cochran's Q test). No significant difference in CMV
       yield was identified using equivalent cell concentrations with inocula
       volumes of 0.2 or 0.4 mL. The CMV sensitivity rate using the higher
       leukocyte inoculum surpassed that obtained by conventional tube
       culture-blind passage. CONCLUSION--These data denote the importance of
       leukocyte concentration on the rate of CMV detection in peripheral blood
       by the shell vial assay-indirect immunofluorescent assay. The data also
       point out the need to establish a standardized blood preparation
       protocol to achieve optimal clinical relevance of this widely used
       laboratory test.
 DE    Antigens, Viral/*ANALYSIS  AIDS-Related Opportunistic
       Infections/*DIAGNOSIS  Cells, Cultured  Centrifugation
       Cytomegalovirus/IMMUNOLOGY/*ISOLATION & PURIF  Cytomegalovirus
       Infections/*DIAGNOSIS  False Negative Reactions  Fluorescent Antibody
       Technique, Indirect  Human  Leukocyte Count  Neutrophils/*VIROLOGY
       Predictive Value of Tests  Reproducibility of Results  Sensitivity and
       Specificity  Support, Non-U.S. Gov't  Viremia/*DIAGNOSIS  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

