       Document 0548
 DOCN  M9640548
 TI    Characterization of postcardiac transplant lymphomas. Histology,
       immunophenotyping, immunohistochemistry, and gene rearrangement.
 DT    9604
 AU    Kowal-Vern A; Swinnen L; Pyle J; Radvany R; Dizikes G; Michalov M;
       Molnar Z; Department of Pathology, Loyola University Medical Center,;
       Maywood, Ill 60153, USA.
 SO    Arch Pathol Lab Med. 1996 Jan;120(1):41-8. Unique Identifier : AIDSLINE
       MED/96136015
 AB    OBJECTIVE--Between 2% and 9% of cardiac transplant recipients develop
       posttransplant lymphoproliferative disease, which includes lymphomas.
       These are usually aggressive Epstein-Barr virus-associated B-cell
       proliferations similar to those seen in other immunodeficiency states. A
       retrospective pathologic study of the tumor tissue from 21 cardiac
       transplant recipients with posttransplant lymphoproliferative disease
       was undertaken. DESIGN--Tumor histology, immunohistochemistry,
       immunophenotyping, and DNA analysis for clonal gene rearrangement and
       the presence of Epstein-Barr virus DNA were performed. PATIENTS--The
       mean patient age was 53.4 +/- 10.2 years (range 33-67 years); 33% of the
       patients were alive at the time of study. RESULTS--Histologically, the
       samples comprised one Burkitt's lymphoma, three diffuse mixed lymphomas,
       eight diffuse large-cell lymphomas, and nine immunoblastic lymphomas.
       Thirteen (93%) of 14 samples were infiltrated by small reactive T cells;
       five of the lymphomas qualified as T-cell rich. Of 14 cases studied, 12
       had clonal immunoglobulin gene rearrangements, 1 had oligoclonal bands,
       and 1 exhibited only a germline pattern. The B cells were CD10+, CD19+,
       and CD20+, and the reactive T cells were CD2+, CD3+, CD5+, CD7+, CD8+,
       and CD57+ by immunophenotyping. CONCLUSIONS--In this patient series,
       morphologically aggressive lymphomas and disseminated disease occurred
       early as well as late after transplantation. Most of the tumors showed a
       reactive T-cell component, which may represent a host attempt at
       controlling the B-cell proliferation.
 DE    Adult  Aged  Antibodies, Monoclonal  Antigens, CD/ANALYSIS
       B-Lymphocytes/IMMUNOLOGY  DNA, Viral/ANALYSIS  Female  *Gene
       Rearrangement  Heart Transplantation/*ADVERSE EFFECTS  Herpesvirus 4,
       Human/GENETICS  Human  *Immunoenzyme Techniques  *Immunophenotyping
       Immunosuppression/ADVERSE EFFECTS  Lymphoma,
       High-Grade/ETIOLOGY/GENETICS/*PATHOLOGY  Lymphoma,
       Intermediate-Grade/ETIOLOGY/GENETICS/*PATHOLOGY  Male  Middle Age
       Retrospective Studies  T-Lymphocytes/IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

