       Document 0557
 DOCN  M9640557
 TI    Diagnosis of microsporidial keratitis by confocal microscopy and the
       chromatrope stain.
 DT    9604
 AU    Shah GK; Pfister D; Probst LE; Ferrieri P; Holland E; Department of
       Ophthalmology, University of Minnesota Hospital; 55455-0501, USA.
 SO    Am J Ophthalmol. 1996 Jan;121(1):89-91. Unique Identifier : AIDSLINE
       MED/96142051
 AB    PURPOSE: To illustrate the value of confocal microscopy and chromatrope
       stain in the diagnosis of microsporidial keratitis. METHODS: In vivo
       confocal microscopy was performed on a man with the human
       immunodeficiency virus who had severe bilateral epithelial keratitis
       refractory to topical antibacterial medications. The results were
       compared to conjunctival scrapings stained with the chromatrope-based
       Weber stain. RESULTS: Confocal microscopy demonstrated many small,
       intraepithelial opacities of the corneal epithelium, which were
       suggestive of Microsporidia. Results of the chromatrope stain of
       conjunctival scrapings confirmed the diagnosis of microsporidial
       keratitis. CONCLUSIONS: Rapid diagnosis allowed prompt initiation of
       topical fumagillin, which permitted rapid, long-term control of the
       symptoms of microsporidial keratitis.
 DE    Adult  Animal  Antiprotozoal Agents/THERAPEUTIC USE  AIDS-Related
       Opportunistic Infections/*DIAGNOSIS/DRUG THERAPY/  PARASITOLOGY  Case
       Report  Conjunctiva/DRUG EFFECTS/PARASITOLOGY  Cornea/DRUG
       EFFECTS/PARASITOLOGY  Dyes  Epithelium/DRUG EFFECTS/PARASITOLOGY  Eye
       Infections, Parasitic/*DIAGNOSIS/DRUG THERAPY/PARASITOLOGY  Fatty Acids,
       Unsaturated/THERAPEUTIC USE  Human  Keratitis/*DIAGNOSIS/DRUG
       THERAPY/PARASITOLOGY  Male  Microscopy, Confocal/*METHODS  Microspora
       Infections/*DIAGNOSIS/DRUG THERAPY/PARASITOLOGY  Microsporida/*ISOLATION
       & PURIF  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

