       Document 0569
 DOCN  M9640569
 TI    Rapid generation of antibodies against the HTLV-II external envelope
       protein by growth of mouse plasmacytomas in SCID mice.
 DT    9604
 AU    Li QX; Camerini D; Kuritzkes DR; Chen IS; Department of Microbiology and
       Immunology, UCLA School of; Medicine 90095, USA.
 SO    Virology. 1995 Dec 20;214(2):680-4. Unique Identifier : AIDSLINE
       MED/96130214
 AB    Recombinant human T-cell leukemia virus type II (HTLV-II) envelope
       external glycoprotein, gp46-II, was expressed using a vaccinia virus
       vector. A recombinant gp46-II fused to an epitope of the influenza virus
       hemagglutinin, YPYDVPDYA, was purified by immunoaffinity chromatography.
       The purified glycoprotein was used to immunize Balb/c mice, and
       antibodies against gp46-II were detected by Western blot analysis and
       syncytium inhibition assays. We transformed spleen cells from the
       immunized mice by retroviral infection with ABL-MYC (psi 2) and
       intraperitoneally transplanted the infected cells into syngeneic Balb/c
       and severe combined immunodeficient (SCID) mice. The plasmacytomas
       established ascitic tumors that produced antibodies directed against
       HTLV-II gp46-II. Ascites developed more rapidly in SCID mice than in
       normal syngeneic mice. This procedure provides a general means to
       generate antibodies rapidly.
 DE    Amino Acid Sequence  Animal  Antibodies, Viral/*BIOSYNTHESIS/IMMUNOLOGY
       Cell Line  Cell Transformation, Viral  Gene Products,
       env/GENETICS/*IMMUNOLOGY  Hemagglutinins, Viral/GENETICS/METABOLISM
       Human  HTLV-BLV Antigens/GENETICS/*IMMUNOLOGY  Mice  Mice, Inbred BALB C
       Mice, SCID  Molecular Sequence Data  Plasmacytoma/*IMMUNOLOGY
       Recombinant Fusion Proteins/IMMUNOLOGY  Retroviridae Proteins,
       Oncogenic/GENETICS/*IMMUNOLOGY  Spleen/CYTOLOGY  Support, Non-U.S. Gov't
       Support, U.S. Gov't, P.H.S.  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

