       Document 0570
 DOCN  M9640570
 TI    Human PKR transfected into murine cells stimulates expression of genes
       under control of the HIV1 or HTLV-I LTR.
 DT    9604
 AU    Meurs EF; McMillan N; Williams BR; Hovanessian AG; Southern PJ;
       Department of Microbiology, University of Minnesota, Minneapolis;
       55455-0312, USA.
 SO    Virology. 1995 Dec 20;214(2):653-9. Unique Identifier : AIDSLINE
       MED/96130209
 AB    We have analyzed the effect of transfection into murine NIH/3T3 cells of
       the human dsRNA-activated kinase PKR on the expression of the
       beta-galactosidase reporter gene, placed under control of the HIV1 or
       the HTLV-I LTR. beta-Galactosidase expression is stimulated when the
       reporter plasmids are cotransfected with wild-type PKR but inhibited
       when cotransfected with a catalytically inactive mutant PKR. In the case
       of HIV1, beta-galactosidase expression was not stimulated when
       cotransfection was carried out with PKR harboring mutations in the dsRNA
       binding domains, indicating that stimulation depends on the classical
       mode of PKR activation through dsRNA binding. In contrast, the dsRNA
       binding mutants of PKR could still partially stimulate
       beta-galactosidase expression from the HTLV-I LTR, suggesting that PKR
       activation in this case may involve different/additional mechanisms.
       These results show that, in addition to the known down-regulation of
       protein synthesis through elF2 phosphorylation, PKR can also positively
       stimulate gene expression in vivo, most probably through phosphorylation
       of a substrate distinct from elF2.
 DE    beta-Galactosidase/GENETICS  Amino Acid Sequence  Animal  Binding Sites
       *Gene Expression Regulation, Enzymologic  *Gene Expression Regulation,
       Viral  Genes, Reporter  Human  HIV Long Terminal Repeat/*GENETICS
       HIV-1/*GENETICS  HTLV-I/*GENETICS  Mice  Molecular Sequence Data
       Mutation  Protein-Serine-Threonine Kinases/GENETICS/*METABOLISM
       Repetitive Sequences, Nucleic Acid/*GENETICS  Structure-Activity
       Relationship  Support, Non-U.S. Gov't  Transfection  3T3 Cells  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

