       Document 0571
 DOCN  M9640571
 TI    The carboxy-terminal domain is essential for stability and not for
       virion incorporation of HIV-1 Vpr into virus particles.
 DT    9604
 AU    Mahalingam S; Patel M; Collman RG; Srinivasan A; Department of
       Microbiology and Immunology, Jefferson Cancer; Institute, Thomas
       Jefferson University, Philadelphia,; Pennsylvania 19107, USA.
 SO    Virology. 1995 Dec 20;214(2):647-52. Unique Identifier : AIDSLINE
       MED/96130208
 AB    Vpr is one of the auxiliary gene products encoded by HIV-1 genome. Vpr
       is a 14-kDa protein and exhibits several interesting characteristics
       including incorporation into virus particles, oligomerization,
       localization in the nucleus, and positive regulation of virus
       replication in primary cells. In an effort to define the
       structure-function relationship of Vpr, the role of the C-terminus of
       Vpr was investigated. Site-specific mutagenesis involving deletion,
       insertion, and substitution of residues at the C-terminus was utilized
       to generate variants of Vpr. Mutations introduced at the C-terminus
       affected properties of Vpr in different ways: (i) Vpr containing amino
       acids 1-72 showed the virion incorporation phenotype, indicating that
       the C-terminus is not essential for this function, (ii) the C-terminus
       contributes to the stability of Vpr, and (iii) substitution mutagenesis
       involving the basic residues showed stability similar to that of wild
       type, indicating the lack of involvement of these residues in this
       biochemical property of Vpr. The data generated in this study and our
       early mutagenic analyses on Vpr suggest that domains noncontiguous in
       primary sequence contribute to the stability of Vpr through overall
       conformation of the protein.
 DE    Amino Acid Sequence  Base Sequence  Binding Sites  Carboxylic Acids  DNA
       Primers  Gene Products, vpr/CHEMISTRY/GENETICS/*METABOLISM  Hela Cells
       Human  HIV-1/*METABOLISM  Molecular Sequence Data  Mutagenesis,
       Site-Directed  Structure-Activity Relationship  Support, Non-U.S. Gov't
       Support, U.S. Gov't, P.H.S.  Virion/METABOLISM  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

