       Document 0576
 DOCN  M9640576
 TI    Effector functions of antibody and CD8+ cells in resolution of rotavirus
       infection and protection against reinfection in mice.
 DT    9604
 AU    McNeal MM; Barone KS; Rae MN; Ward RL; Division of Clinical Virology,
       James N. Gamble Institute of; Medical Research, Cincinnati, Ohio 45219,
       USA.
 SO    Virology. 1995 Dec 20;214(2):387-97. Unique Identifier : AIDSLINE
       MED/96130177
 AB    The importance of antibody and CD8+ cells in resolution of murine
       rotavirus (EDIM) infection and protection against reinfection was
       examined with two strains of B-cell-deficient mice. Following
       inoculation of one strain (JHD), rotavirus infection was resolved within
       days, but when later reinoculated with EDIM, these mice again shed
       rotavirus. Thus, effector mechanisms other than antibody resolved viral
       shedding in JHD mice but were insufficient to prevent reinfection. EDIM
       shedding in another B-cell-deficient mouse strain (microMT) diminished
       but was not fully resolved 93 days after the initial infection, thus
       demonstrating that antibody could also be important in resolution of
       rotavirus infection. When depleted of CD8+ cells by monoclonal antibody
       treatment before EDIM inoculation, JHD mice were unable to resolve
       shedding. Even though microMT mice did not fully resolve their initial
       infection, depletion of CD8+ cells 49 days after initial inoculation
       resulted in a burst of shedding. Thus, CD8+ cells were involved in
       resolution of the initial EDIM infection in both strains of
       B-cell-deficient mice. Finally, when microMT mice were depleted of CD8+
       cells before the initial EDIM infection, gradual resolution of rotavirus
       shedding was still observed, suggesting a third effector mechanism was
       also involved in resolution of rotavirus infection in mice.
 DE    Animal  Antibodies, Viral/*IMMUNOLOGY  CD8-Positive
       T-Lymphocytes/*IMMUNOLOGY  Disease Models, Animal  Feces/VIROLOGY
       Female  Gene Deletion  Immunoglobulins, mu-Chain/GENETICS/IMMUNOLOGY
       Immunoglobulins, Heavy-Chain/GENETICS/IMMUNOLOGY  Mice  Mice, Inbred
       BALB C  Mice, Inbred C57BL  Mice, Mutant Strains
       Rotavirus/*IMMUNOLOGY/ISOLATION & PURIF  Rotavirus
       Infections/*IMMUNOLOGY/PREVENTION & CONTROL/VIROLOGY  Support, U.S.
       Gov't, P.H.S.  Virus Shedding  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

