       Document 0599
 DOCN  M9640599
 TI    Specific interferon-gamma producing CD4+ and CD8+ T cells after
       autologous EBV-B stimulation: the necessity of restimulation.
 DT    9604
 AU    Vooijs WC; Nootebos MG; Wernert H; de Gast GC; Department of
       Immunohaematology, University Hospital Utrecht, The; Netherlands.
 SO    Scand J Immunol. 1995 Dec;42(6):591-7. Unique Identifier : AIDSLINE
       MED/96150308
 AB    The response of T cells to produce interferon-gamma, to proliferate and
       to become cytotoxic after specific stimulation with low dose (2%)
       autologous EBV-B cells was investigated in 15 EBV seropositive and five
       seronegative patients. A significantly higher number of interferon-gamma
       producing cells (56 +/- 24 per 10(5) T cells) were found in a spot ELISA
       in EBV positive than in EBV negative patients (7 +/- 2 spots, P < 0.01)
       and it was only found with restimulation after 5-12 days of primary
       culture. No correlation was found between the extent of interferon-gamma
       production, cytotoxicity or proliferation. Specificity of EBV-induced
       interferon-gamma production was demonstrated by comparison of the
       response to allogeneic EBV-B cells or IL-2 in the restimulation phase.
       The response was stronger in CD8+ T cells than in CD4+ T cells and could
       be blocked in the restimulation phase with HLA class I and class II
       antiserum, respectively.
 DE    Cytotoxicity, Immunologic  CD4-Positive T-Lymphocytes/*IMMUNOLOGY
       CD8-Positive T-Lymphocytes/*IMMUNOLOGY  Herpesvirus 4, Human/*IMMUNOLOGY
       Human  HLA Antigens/IMMUNOLOGY  In Vitro  Interferon Type
       II/*BIOSYNTHESIS  *Lymphocyte Transformation  Time Factors  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

