       Document 0608
 DOCN  M9640608
 TI    Inhibition of human immunodeficiency virus type 1 and vaccinia virus
       infection by a dominant negative factor of the interferon regulatory
       factor family expressed in monocytic cells.
 DT    9604
 AU    Thornton AM; Buller RM; DeVico AL; Wang IM; Ozato K; Laboratory of
       Molecular Growth Regulation, National Institute of; Child Health and
       Human Development, National Institutes of; Health, Bethesda, MD 20892,
       USA.
 SO    Proc Natl Acad Sci U S A. 1996 Jan 9;93(1):383-7. Unique Identifier :
       AIDSLINE MED/96133941
 AB    ICSBP is a member of the interferon (IFN) regulatory factor (IRF) family
       that regulates expression of type I interferon (IFN) and IFN-regulated
       genes. To study the role of the IRF family in viral infection, a cDNA
       for the DNA-binding domain (DBD) of ICSBP was stably transfected into
       U937 human monocytic cells. Clones that expressed DBD exhibited a
       dominant negative phenotype and did not elicit antiviral activity
       against vesicular stomatitis virus (VSV) infection upon IFN treatment.
       Most notably, cells expressing DBD were refractory to infection by
       vaccinia virus (VV) and human immunodeficiency virus type 1 (HIV-1). The
       inhibition of VV infection was attributed to defective virion assembly,
       and that of HIV-1 to low CD4 expression and inhibition of viral
       transcription in DBD clones. HIV-1 and VV were found to have sequences
       in their regulatory regions similar to the IFN-stimulated response
       element (ISRE) to which IRF family proteins bind. Accordingly, these
       viral sequences and a cellular ISRE bound a shared factor(s) expressed
       in U937 cells. These observations suggest a novel host-virus
       relationship in which the productive infection of some viruses is
       regulated by the IRF-dependent transcription pathway through the ISRE.
 DE    Base Sequence  Carrier Proteins/*PHYSIOLOGY  Cells, Cultured
       DNA-Binding Proteins/METABOLISM  *Gene Expression Regulation, Viral
       Genes, Dominant  Human  HIV Infections/*PREVENTION & CONTROL
       HIV-1/*GENETICS  Interferons/PHYSIOLOGY  Molecular Sequence Data
       Oligodeoxyribonucleotides/CHEMISTRY  Regulatory Sequences, Nucleic Acid
       Repressor Proteins/*GENETICS/METABOLISM  Vaccinia/*PREVENTION & CONTROL
       Vaccinia Virus/*GENETICS  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

