       Document 0622
 DOCN  M9640622
 TI    Resolution of cryptosporidial infection in mice correlates with
       parasite-specific lymphocyte proliferation associated with both Th1 and
       Th2 cytokine secretion.
 DT    9604
 AU    Tilley M; McDonald V; Bancroft GJ; London School of Hygiene and Tropical
       Medicine, Department of; Clinical Sciences, UK.
 SO    Parasite Immunol. 1995 Sep;17(9):459-64. Unique Identifier : AIDSLINE
       MED/96143612
 AB    This study was designed to investigate and characterize T-cell responses
       which lead to elimination of a primary infection of Cryptosporidium
       muris in BALB/c mice. The proliferative response of spleen cells to
       parasite antigen was measured by uptake of 3H-thymidine and, in
       parallel, supernatants were removed from cells to measure levels of
       IFN-gamma, TNF, IL-2 and IL-4 by ELISA. Oocyst excretion in faeces was
       first detected on day 10 post infection (p.i.); the level of shedding
       subsequently increased until day 14 and then declined until no oocysts
       were detected by day 25. The proliferative response of spleen cells from
       infected animals was similar to control levels up to day 14 p.i. but
       increased significantly on day 21 and was even greater on day 26.
       IFN-gamma and IL-2 were detected initially on day 14 p.i. and
       significantly higher concentrations were found on days 21 and 26. IL-4
       secretion was also detected, but not until day 21 p.i., and production
       of TNF was not found at any time. Depletion of T-cells or CD4+ cells
       from spleen cells cultured with antigen resulted in a significant
       decrease in the levels of cytokine detected. These results indicated,
       therefore, that in BALB/c mice there was a correlation between the
       development of immunity to C. muris infection and both a parasite
       antigen-specific proliferative response and Th1 and Th2 cytokine
       production by spleen cells.
 DE    Animal  Cryptosporidiosis/*IMMUNOLOGY  Cryptosporidium/IMMUNOLOGY
       Cytokines/*SECRETION  Interferon Type II/SECRETION
       Interleukin-2/SECRETION  Interleukin-4/SECRETION  *Lymphocyte
       Transformation  Mice  Mice, Inbred BALB C  Support, Non-U.S. Gov't  Th1
       Cells/*IMMUNOLOGY  Th2 Cells/*IMMUNOLOGY  Tumor Necrosis
       Factor/SECRETION  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

