       Document 0623
 DOCN  M9640623
 TI    Interaction of ETS-1 and ERGB/FLI-1 proteins with DNA is modulated by
       spacing between multiple binding sites as well as phosphorylation.
 DT    9604
 AU    Hodge DR; Robinson L; Watson D; Lautenberger J; Zhang XK; Venanzoni M;
       Seth A; Laboratory of Molecular Oncology, National Cancer Institute,;
       Frederick, MD 21702-1201, USA.
 SO    Oncogene. 1996 Jan 4;12(1):11-8. Unique Identifier : AIDSLINE
       MED/96140822
 AB    ETS is a family of transcription factors that contain a highly conserved
       ETS DNA binding domain. Various members of the ETS family are expressed
       in cells of hematopoietic lineage. ETS-1, ETS-2 and ERGB/FLI-1 are
       expressed at high levels in T-lymphocytes. HIV-1 infects T-cells and it
       has been shown that its LTR contains binding sites for various
       transcription factors. In this study we show that the HIV-1 core
       enhancer is directly regulated by ERGB/FLI-1 protein positively, as well
       as, negatively, depending upon the presence or absence of accessory
       factors in different cell types. In addition, we show that the ETS-1
       transactivation activity is enhanced upon dephosphorylation of the
       Calmodulin-dependent Protein Kinase II phosphorylation site located in
       exon VII. Finally, we demonstrate that the spacing between the two EBS
       cores in palindromic or direct repeat sites play a crucial role in
       binding of ETS proteins to DNA.
 DE    Animal  Base Sequence  Binding Sites  DNA/*METABOLISM  DNA-Binding
       Proteins/*METABOLISM  Enhancer Elements (Genetics)  HIV Long Terminal
       Repeat  Molecular Sequence Data  Phosphorylation  Proto-Oncogene
       Proteins/*METABOLISM  Spodoptera  Support, Non-U.S. Gov't
       Trans-Activators/*METABOLISM  Transcription Factors/*METABOLISM  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

