       Document 0641
 DOCN  M9640641
 TI    PCR-in situ hybridization detection of human T-cell lymphotropic virus
       type 1 (HTLV-1) tax proviral DNA in peripheral blood lymphocytes of
       patients with HTLV-1-associated neurologic disease.
 DT    9604
 AU    Levin MC; Fox RJ; Lehky T; Walter M; Fox CH; Flerlage N; Bamford R;
       Jacobson S; Neuroimmunology Branch, National Institute of Neurological;
       Diseases and Stroke, NIH, Bethesda, MD 20852, USA.
 SO    J Virol. 1996 Feb;70(2):924-33. Unique Identifier : AIDSLINE
       MED/96135203
 AB    PCR-in situ hybridization (PCR-ISH) was developed and utilized to
       determine the distribution of human T-cell lymphotropic virus type 1
       (HTLV-1) tax proviral DNA in peripheral blood lymphocytes (PBL) from
       patients with HTLV-1-associated myelopathy/tropical spastic paraparesis
       (HAM/TSP). PCR-ISH of HTLV-1 tax DNA in PBL from patients with HAM/TSP
       revealed that 1 in 5,000 to 1 in 10,000 PBL contained virus. PCR-ISH was
       sensitive, because a positive signal was consistently demonstrated from
       the HTLV-1-infected cell lines HUT-102 (which contains four to six
       copies of HTLV-1 proviral DNA per cell) and MT-1 (which contains one to
       three copies of HTLV-1 proviral DNA per cell). Also, intracellular
       amplification by PCR-ISH significantly increased sensitivity compared
       with conventional ISH and was shown to be specific for HTLV-1 tax DNA.
       These results are in contrast to solution-phase PCR amplification in
       which greater than 1% of cells were estimated to be infected. The
       discordance between these results is discussed and may indicate that
       more than one copy of HTLV-1 tax proviral DNA is present in an
       individual PBL.
 DE    Base Sequence  Cell Line  DNA Primers  DNA, Viral/GENETICS/*ISOLATION &
       PURIF  Gene Products, tax/*GENETICS  Human  HTLV-I/*ISOLATION & PURIF
       In Situ Hybridization  Lymphocytes/VIROLOGY  Molecular Sequence Data
       Paraparesis, Tropical Spastic/BLOOD/*VIROLOGY  Polymerase Chain
       Reaction/*METHODS  Proviruses/GENETICS/ISOLATION & PURIF  Sensitivity
       and Specificity  Support, Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

