       Document 0642
 DOCN  M9640642
 TI    Neutrophil-mediated suppression of virus replication after herpes
       simplex virus type 1 infection of the murine cornea.
 DT    9604
 AU    Tumpey TM; Chen SH; Oakes JE; Lausch RN; Department of Microbiology and
       Immunology, College of Medicine,; University of South Alabama, Mobile
       36688, USA.
 SO    J Virol. 1996 Feb;70(2):898-904. Unique Identifier : AIDSLINE
       MED/96135200
 AB    Herpes simplex virus type 1 (HSV-1) infection of the murine cornea
       induces the rapid infiltration of neutrophils. We investigated whether
       these cells could influence virus replication. BALB/c mice treated with
       monoclonal antibody (MAb) RB6-8C5 experienced a profound depletion of
       neutrophils in the bloodstream, spleen, and cornea. In these animals,
       virus titers in the eye were significantly higher than those in the
       immunoglobulin G-treated controls at 3 days postinfection. By day 9,
       virus was no longer detectable in the controls, whereas titers of 10(3)
       to 10(6) PFU were still present in the neutrophil-depleted hosts.
       Furthermore, virus spread more readily to the skin and brains of MAb
       RB6-8C5-treated animals, rendering them significantly more susceptible
       to HSV-1-induced blepharitis and encephalitis. Only 25% of the treated
       animals survived, whereas all of the controls lived. Although MAb
       RB6-8C5 treatment did not alter the CD4+ T-cell, B-cell, natural killer
       cell, or macrophage populations, the CD8+ T-cell population was
       partially reduced. Therefore, the experiments were repeated in severe
       combined immunodeficiency mice, which lack CD8+ T cells. Again virus
       growth was found to be significantly elevated in the eyes, trigeminal
       ganglia, and brains of the MAb RB6-8C5-treated hosts. These results
       strongly indicate that in both immunocompetent and immunodeficient mice,
       neutrophils play a significant role in helping to control the
       replication and spread of HSV-1 after corneal infection.
 DE    Animal  Antibodies, Monoclonal/IMMUNOLOGY
       Blepharitis/IMMUNOLOGY/VIROLOGY  Cercopithecus aethiops  CD8-Positive
       T-Lymphocytes/IMMUNOLOGY  Eye/VIROLOGY  Female  Herpesvirus 1,
       Human/GROWTH & DEVELOPMENT/*IMMUNOLOGY  Human  Keratitis,
       Herpetic/*IMMUNOLOGY/VIROLOGY  Leukocyte Count  Mice  Mice, Inbred BALB
       C  Mice, SCID  Neutrophils/*IMMUNOLOGY  Support, U.S. Gov't, P.H.S.
       Vero Cells  *Virus Replication  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

