       Document 0643
 DOCN  M9640643
 TI    An array of murine leukemia virus-related elements is transmitted and
       expressed in a primate recipient of retroviral gene transfer.
 DT    9604
 AU    Purcell DF; Broscius CM; Vanin EF; Buckler CE; Nienhuis AW; Martin MA;
       Laboratory of Molecular Microbiology, National Institute of; Allergy and
       Infectious Diseases, National Institutes of Health,; Bethesda, MD 20892,
       USA.
 SO    J Virol. 1996 Feb;70(2):887-97. Unique Identifier : AIDSLINE
       GENBANK/K01384
 AB    Direct RNA-PCR analyses of T-cell lymphomas that developed in rhesus
       macaques during a gene transfer experiment revealed the presence of
       several different recombinant murine leukemia viruses (MuLV). Most
       prominent was the expected MuLV recombinant, designated MoLTRAmphoenv in
       which the amphotropic env of the helper packaging virus was joined to
       the long terminal repeat (LTR) of the Moloney MuLV-derived vector. This
       retrovirus does not exist in nature. An additional copy of the core
       enhancer acquired from the vector LTR may have augmented the replicative
       properties of MoLTRAmphoenv MuLV in several different rhesus cell types
       compared with the prototype amphotropic MuLV4070A. Unexpectedly, at
       least two types of mink cell focus-forming MuLV elements, arising from
       endogenous retroviral sequences expressed in the murine packaging cell
       line, were also transmitted and highly expressed in one of the macaques.
       Furthermore, murine virus-like VL-30 sequences were detected in the
       rhesus lymphomas, but these were not transcribed into RNA. The
       unanticipated presence of an array of MuLV-related structures in a
       primate gene transfer recipient demands ever-vigilant scrutiny for the
       existence of transmissible retroviral elements and replication-competent
       viruses possessing altered tropic or growth properties in packaging
       cells producing retroviral vectors.
 DE    Animal  Base Sequence  Cells, Cultured  Cloning, Molecular  DNA, Viral
       Gene Expression Regulation, Viral  Gene Transfer/*ADVERSE EFFECTS
       Genes, env  *Genetic Vectors  Leukemia Viruses,
       Murine/*GENETICS/ISOLATION & PURIF/  PATHOGENICITY  Lymphoma/*VIROLOGY
       Macaca mulatta  Mice  Molecular Sequence Data  Promoter Regions
       (Genetics)  Repetitive Sequences, Nucleic Acid  Retroviridae
       Infections/*VIROLOGY  Support, Non-U.S. Gov't  Transcription, Genetic
       Tumor Virus Infections/*VIROLOGY  Viral Envelope Proteins/GENETICS
       Virus Replication  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

