       Document 0644
 DOCN  M9640644
 TI    trans-acting proteins involved in RNA encapsidation and viral assembly
       in human immunodeficiency virus type 1.
 DT    9604
 AU    Kaye JF; Lever AM; Department of Medicine, Addenbrooke's Hospital,
       Cambridge, United; Kingdom.
 SO    J Virol. 1996 Feb;70(2):880-6. Unique Identifier : AIDSLINE MED/96135198
 AB    The human immunodeficiency virus type 1 gag gene product Pr55gag
       self-assembles when expressed on its own in a variety of eukaryotic
       systems. Assembly in T lymphocytes has not previously been studied, nor
       is it clear whether Pr55gag particles can package genomic RNA or if the
       Gag-Pol polyprotein is required. We have used a series of constructs
       that express Gag or Gag-Pol proteins with or without the viral protease
       in transient transfections in COS-1 cells and also expressed stably in
       CD4+ T cells to study this. Deletion of the p6 domain at the C terminus
       of protease-negative Pr55gag did not abolish particle release, while
       truncation of the nucleocapsid protein reduced it significantly,
       particularly in lymphocytes. Gag-Pol polyprotein was released from T
       cells in the absence of Pr55gag but did not encapsidate RNA. Pr55gag
       encapsidated human immunodeficiency virus type 1 RNA whether expressed
       in a protease-positive or protease-negative context. p6 was dispensable
       for RNA encapsidation. Marked differences in the level of RNA export
       were noted between the different cell lines.
 DE    Amino Acid Sequence  Animal  Binding Sites  Cell Line  Gene Products,
       gag/GENETICS/*PHYSIOLOGY  Gene Products, pol/GENETICS/PHYSIOLOGY  Genes,
       gag  Human  HIV-1/GENETICS/*PHYSIOLOGY  Molecular Sequence Data
       Mutation  Protein Precursors/GENETICS/*PHYSIOLOGY  RNA,
       Viral/*METABOLISM  Support, Non-U.S. Gov't
       Trans-Activators/GENETICS/*PHYSIOLOGY  Virion/METABOLISM  Virus
       Assembly/*PHYSIOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

