       Document 0656
 DOCN  M9640656
 TI    Nuclear preservation and cytoplasmic degradation of human
       immunodeficiency virus type 1 Rev protein.
 DT    9604
 AU    Kubota S; Duan L; Furuta RA; Hatanaka M; Pomerantz RJ; Dorrance H.
       Hamilton Laboratories, Department of Medicine,; Jefferson Medical
       College, Thomas Jefferson University,; Philadelphia, Pennsylvania, USA.
 SO    J Virol. 1996 Feb;70(2):1282-7. Unique Identifier : AIDSLINE
       MED/96135251
 AB    Rev, a major regulatory protein of human immunodeficiency virus type 1,
       has been demonstrated to shuttle between the nucleus and cytoplasm of
       infected cells. The fate of the Rev protein in living cells was
       evaluated by pulse-chase experiments using a transient Rev expression
       system. Sixteen hours after chasing with unlabelled amino acids, 45% of
       the labelled Rev was still present, which clearly indicates a long
       half-life of Rev in living cells. A Rev mutant which is deficient in the
       ability to migrate from the nucleus to the cytoplasm was degraded more
       slowly than the wild-type Rev protein. As well, another Rev mutant
       protein, which lacks a functional nucleolar targeting signal (NOS) and
       is unable to enter the cell nucleus, was rapidly degraded and
       undetectable 16 h after chasing. Nuclear-nucleolar targeting properties
       provided by a divergent NOS from a related retrovirus, which was used to
       substitute for the NOS of Rev, increased the intracellular half-life of
       this Rev mutant. Moreover, coexpression of an intracellular anti-Rev
       single-chain antibody (SFv), which has been shown to interfere with the
       nuclear translocation of Rev, accelerated the degradation of the
       wild-type Rev protein. Differential degradation of Rev in the nucleus
       and cytoplasm may play a critical role in determining and maintaining
       different stages of human immunodeficiency virus type 1 infection, in
       conjunction with the shuttling properties of the Rev protein.
 DE    Amino Acid Sequence  Animal  Antibodies, Monoclonal/METABOLISM  Cell
       Line, Transformed  Cell Nucleus/METABOLISM  Chimeric Proteins/METABOLISM
       Cytoplasm/METABOLISM  Gene Products, rev/*METABOLISM  Gene Products,
       rex/GENETICS/METABOLISM  Half-Life  Human  HIV-1/*METABOLISM  Molecular
       Sequence Data  Mutation  Recombinant Fusion Proteins/GENETICS/METABOLISM
       Support, U.S. Gov't, P.H.S.  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

