       Document 0657
 DOCN  M9640657
 TI    Human monoclonal antibody 2G12 defines a distinctive neutralization
       epitope on the gp120 glycoprotein of human immunodeficiency virus type
       1.
 DT    9604
 AU    Trkola A; Purtscher M; Muster T; Ballaun C; Buchacher A; Sullivan N;
       Srinivasan K; Sodroski J; Moore JP; Katinger H; Aaron Diamond AIDS
       Research Center, New York University School of; Medicine, New York
       10016, USA.
 SO    J Virol. 1996 Feb;70(2):1100-8. Unique Identifier : AIDSLINE
       MED/96135224
 AB    We have isolated and characterized human monoclonal antibody 2G12 to the
       gp120 surface glycoprotein of human immunodeficiency virus type 1
       (HIV-1). This antibody potently and broadly neutralizes primary and
       T-cell line-adapted clade B strains of HIV-1 in a peripheral blood
       mononuclear cell-based assay and inhibits syncytium formation in the
       AA-2 cell line. Furthermore, 2G12 possesses neutralizing activity
       against strains from clade A but not from clade E. Complement- and
       antibody-dependent cellular cytotoxicity-activating functions of 2G12
       were also defined. The gp120 epitope recognized by 2G12 was found to be
       distinctive; binding of 2G12 to LAI recombinant gp120 was abolished by
       amino acid substitutions removing N-linked carbohydrates in the C2, C3,
       V4, and C4 regions of gp120. This gp120 mutant recognition pattern has
       not previously been observed, indicating that the 2G12 epitope is
       unusual. consistent with this, antibodies able to block 2G12 binding to
       recombinant gp120 were not detected in significant quantities in 16
       HIV-positive human serum samples.
 DE    Amino Acid Sequence  Antibodies, Monoclonal/*IMMUNOLOGY
       Antibody-Dependent Cell Cytotoxicity/IMMUNOLOGY  Cell Line  Complement
       3/IMMUNOLOGY  *Epitope Mapping  Giant Cells/IMMUNOLOGY/VIROLOGY
       Glycosylation  Human  HIV Antibodies/*IMMUNOLOGY  HIV Envelope Protein
       gp120/*IMMUNOLOGY  HIV-1/*IMMUNOLOGY/ISOLATION & PURIF  Molecular
       Sequence Data  Mutation  Neutralization Tests
       Polysaccharides/IMMUNOLOGY  Support, Non-U.S. Gov't  Support, U.S.
       Gov't, P.H.S.  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

