       Document 0668
 DOCN  M9640668
 TI    Short-term stability of HIV provirus levels in the peripheral blood of
       HIV-infected individuals.
 DT    9604
 AU    Paul DB; Kuhns MC; McNamara AL; Pottage JC Jr; Spear GT; Department of
       Immunology/Microbiology, Rush Presbyterian St.; Luke's Medical Center,
       Chicago, IL 60612, USA.
 SO    J Med Virol. 1995 Nov;47(3):292-7. Unique Identifier : AIDSLINE
       MED/96129559
 AB    Changes in viral load have been reported to reflect disease progression
       or response to therapy; however, the stability of HIV DNA levels in
       HIV-infected individuals has not been extensively studied. Cellular HIV
       DNA levels in infected individuals were evaluated over a short time
       period to determine degree of variability as well as any correlation
       with other measurements of virus load or immune status. Peripheral blood
       mononuclear cells (PBMC) were obtained several times over 1 month from
       32 asymptomatic or symptomatic non-AIDS, HIV-infected individuals
       currently on AZT therapy. PCR amplification of the HIV gag region was
       performed with DNA from PBMC lysates and the PCR amplified products
       quantitated by liquid phase hybridization. HIV DNA levels in the
       majority of the patients were relatively stable, with 26 of 32 persons
       having less than threefold change. Changes over the study period were
       both positive and negative, and the median change in HIV DNA levels was
       68.6%. These changes were found to positively correlate with
       fluctuations in plasma p24 levels. In contrast, no correlations were
       found with other measurements of immune system activity, including
       changes in CD4 number, CD4 percent, and beta 2-microglobulin when
       compared with provirus changes. This study shows that levels of HIV DNA
       can be relatively stable over short periods in most non-AIDS,
       HIV-infected persons.
 DE    beta 2-Microglobulin/ANALYSIS  Base Sequence  Disease Progression  DNA
       Primers  DNA, Viral/ISOLATION & PURIF  Human  HIV Core Protein p24/BLOOD
       HIV Infections/BLOOD/*VIROLOGY  HIV Seropositivity/VIROLOGY
       HIV-1/GENETICS/*ISOLATION & PURIF  Leukocytes, Mononuclear/VIROLOGY
       Molecular Sequence Data  Proviruses/GENETICS/*ISOLATION & PURIF
       Support, Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.  Time Factors
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

