       Document 0671
 DOCN  M9640671
 TI    Hepatitis C virus infection in an HIV-positive population in Normandy:
       antibodies, HCV RNA and genotype prevalence.
 DT    9604
 AU    Mendel I; Clotteau L; Lambert S; Buffet-Janvresse C; Virology
       Department, Charles Nicolle Hospital, University of; Rouen, France.
 SO    J Med Virol. 1995 Nov;47(3):231-6. Unique Identifier : AIDSLINE
       MED/96129550
 AB    The prevalence and the characteristics of hepatitis C virus infection
       (HCV) in 161 HIV-positive patients were studied. HCV seroprevalence was
       determined by enzyme immunoassay and recombinant immunoblot assay
       (RIBA). Two different reverse transcriptase polymerase chain reaction
       (RT-PCR) methods were also used to test the HCV-seropositive samples and
       50 EIA-negative sera used as controls. The RNA HCV-positive sera were
       genotyped by the LiPA procedure. Associations of HCV status with
       demographic characteristics and risk factors were assessed by chi 2 and
       Fisher's exact tests. The seroprevalence of HCV was 34.2% with a
       significant difference between blood and sexual exposure risk groups
       (60.6% vs. 13.6%, respectively; P < 0.0001). Thirty-six of the 55
       anti-HCV-positive sera were also positive for HCV RNA, and PCR detected
       HCV RNA in 8 HCV-seronegative patients. Various RIBA profiles were found
       and all sera were positive for antibodies to the c33 protein. A
       proportion of sera had elevated levels of transaminase activity (37.2%),
       and abnormal liver function as associated with HCV infection. Forty-two
       samples were genotyped and five genotypes and subtypes of the HCV virus
       were detected. Genotype 1a was the most frequent in this cohort,
       although genotype 1b is generally more common in France. The majority
       (94.1%) of the patients with genotype 1a had a history of blood
       exposure, which may account for the difference.
 DE    Adolescence  Adult  Aged  Alanine Aminotransferase/BLOOD  Female
       France/EPIDEMIOLOGY  Genotype  Hepatitis C/*COMPLICATIONS/EPIDEMIOLOGY
       Hepatitis C Antibodies/BLOOD  Hepatitis C Viruses/GENETICS/IMMUNOLOGY
       Human  HIV Seropositivity/*COMPLICATIONS/EPIDEMIOLOGY/IMMUNOLOGY
       IgG/BLOOD  IgM/BLOOD  Immunoblotting  Male  Middle Age  Prevalence  RNA,
       Viral/ANALYSIS  Viremia  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

