       Document 0673
 DOCN  M9640673
 TI    Comparison of human immunodeficiency virus biological phenotypes
       isolated from cerebrospinal fluid and peripheral blood.
 DT    9604
 AU    Peeters MF; Colebunders RL; Van den Abbeele K; Nys PC; Goeman J; Colans
       P; Geerts Y; Van der Groen G; Department of Infection and Immunity,
       University Hospital; Antwerp, Belgium.
 SO    J Med Virol. 1995 Sep;47(1):92-6. Unique Identifier : AIDSLINE
       MED/96048328
 AB    Quantitative human immunodeficiency virus (HIV) cultures were carried
       out on cerebrospinal fluid (CSF), peripheral blood mononuclear cells
       (PBMCs), and plasma from patients with HIV in order to compare the
       infectious HIV load. The HIV strains isolated were studied for
       syncytium-inducing (SI) capacity, using the MT-2 cell line, in order to
       compare the HIV strain phenotype of blood and CSF isolates. Forty-two
       patients with HIV-1 infection were enrolled in the study, 33 of whom had
       neurological symptoms and 9 of whom were without neurological symptoms.
       HIV was isolated from 16 (38%) of the 42 CSF cultures, with a low mean
       titer of 6.3 +/- 3.4 tissue-culture-infective doses (TCID) per
       milliliter. Patients with HIV-positive CSF culture had a viral load in
       PBMCs of 40.5 +/- 15.5 TCID per 10(6) PBMC and in plasma of 104.7 +/-
       9.3 per milliliter. Two (15%) of the 13 CSF isolates were SI strains,
       compared to 17 (56.6%) of the 30 PBMC isolates and 13 (54%) of the 24
       plasma isolates (P < 0.05). Five of the nine patients from whom CSF and
       blood strains were obtained had the same viral biological phenotype.
       This study suggests that different HIV variants may be found in
       different body fluids and/or cells.
 DE    Adult  Cells, Cultured  Comparative Study  Female  Giant Cells/VIROLOGY
       Human  HIV Infections/BLOOD/CEREBROSPINAL FLUID/*VIROLOGY
       HIV-1/*ISOLATION & PURIF/PHYSIOLOGY  Leukocytes, Mononuclear/VIROLOGY
       Male  Neurologic Examination  Phenotype  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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