       Document 0676
 DOCN  M9640676
 TI    Use of the polymerase chain reaction to detect genomes of human
       immunodeficiency virus and cytomegalovirus in post-mortem tissues.
 DT    9604
 AU    Webster A; McLaughlin JE; Johnson MA; Emery VC; Griffiths PD; Division
       of Pathology and Communicable Diseases, Royal Free; Hospital School of
       Medicine, London, United Kingdom.
 SO    J Med Virol. 1995 Sep;47(1):23-8. Unique Identifier : AIDSLINE
       MED/96048317
 AB    The polymerase chain reaction (PCR) was used to amplify a 149 base-pair
       region of the cytomegalovirus (CMV) genome and a 551 base-pair region of
       the HIV-1 proviral long terminal repeat (LTR) present in DNA extracted
       from post-mortem tissue. Multiple tissues (n = 116) obtained from 16
       patients which were subjected to PCR were also subjected to cell culture
       and histopathological analyses. One hundred and seven samples (92%)
       contained CMV DNA and 66/116 (57%) contained HIV proviral DNA at a level
       of > or = 10 genomes. Both viruses were detected in 60/116 (51.7%) of
       samples, with co-infection most frequent in the lung (69%). Cell culture
       for CMV detected 9.3% of the PCR-positive samples, whilst histology
       identified CMV inclusions in 15.9% of samples, all of which were CMV
       PCR-positive. CMV was most frequently detected in adrenal and lung
       tissues by histology. These results show that co-infection with CMV and
       HIV is a common occurrence in organs from AIDS patients and provide
       further evidence for a role of cytomegalovirus in the pathogenesis of
       AIDS.
 DE    AIDS-Related Opportunistic Infections/*VIROLOGY  Base Sequence  Cells,
       Cultured  Cytomegalovirus/GENETICS/*ISOLATION & PURIF  Cytomegalovirus
       Infections/COMPLICATIONS/*VIROLOGY  DNA, Viral/ANALYSIS  Genome, Viral
       Human  HIV/GENETICS/*ISOLATION & PURIF  HIV Long Terminal Repeat
       Molecular Sequence Data  *Polymerase Chain Reaction  Postmortem Changes
       Proviruses/GENETICS  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

