       Document 0686
 DOCN  M9640686
 TI    Cellular targets of infection and route of viral dissemination after an
       intravaginal inoculation of simian immunodeficiency virus into rhesus
       macaques.
 DT    9604
 AU    Spira AI; Marx PA; Patterson BK; Mahoney J; Koup RA; Wolinsky SM; Ho DD;
       Aaron Diamond AIDS Research Center, New York University School of;
       Medicine, New York 10016, USA.
 SO    J Exp Med. 1996 Jan 1;183(1):215-25. Unique Identifier : AIDSLINE
       MED/96136764
 AB    We used the simian immunodeficiency virus (SIV)/rhesus macaque model to
       study events that underlie sexual transmission of human immunodeficiency
       virus type 1 (HIV-1). Four female rhesus macaques were inoculated
       intravaginally with SIVmac251, and then killed 2, 5, 7, and 9 d later. A
       technique that detected polymerase chain reaction-amplified SIV in situ
       showed that the first cellular targets for SIV were in the lamina
       propria of the cervicovaginal mucosa, immediately subjacent to the
       epithelium. Phenotypic and localization studies demonstrated that many
       of the infected cells were likely to be dendritic cells. Within 2 d of
       inoculation, infected cells were identified in the paracortex and
       subcapsular sinus of the draining internal iliac lymph nodes.
       Subsequently, systemic dissemination of SIV was rapid, since culturable
       virus was detectable in the blood by day 5. From these results, we
       present a model for mucosal transmission of SIV and HIV-1.
 DE    Animal  Autopsy/VETERINARY  Base Sequence  Cell Fusion  Cervix
       Uteri/*VIROLOGY  Dendritic Cells/VIROLOGY  Disease Models, Animal
       Female  Histocytochemistry  HIV Infections  HIV-1  Lymphatic
       System/VIROLOGY  Macaca mulatta  Molecular Sequence Data  Mucous
       Membrane/CYTOLOGY/VIROLOGY  Polymerase Chain Reaction  Simian Acquired
       Immunodeficiency Syndrome/TRANSMISSION/*VIROLOGY  Support, Non-U.S.
       Gov't  Support, U.S. Gov't, P.H.S.  SIV/ISOLATION & PURIF  Time Factors
       Vagina/*VIROLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

