       Document 0724
 DOCN  M9640724
 TI    B7.1 expression on tumor cells circumvents the need of professional
       antigen presentation for in vitro propagation of cytotoxic T cell lines.
 DT    9604
 AU    Iezzi G; Protti MP; Rugarli C; Bellone M; Laboratorio I.A., Istituto
       Scientifico H San Raffaele, Milano,; Italy.
 SO    Cancer Res. 1996 Jan 1;56(1):11-5. Unique Identifier : AIDSLINE
       MED/96124868
 AB    In vitro propagation of tumor-specific CTLs, to be used for
       identification of tumor antigens (Ag) and/or adoptive immunotherapy, is
       hampered by the need of large amounts of professional antigen-presenting
       cells (APC) used for periodical cycles of restimulation. We evaluated
       whether RMA T lymphoma cells, stably transfected with the cDNA encoding
       for the B7.1 costimulatory molecule, provided the activation signals to
       CD8+ T lymphocytes in the absence of professional APC and CD4+ helper
       cells. We demonstrate here that long-term CD8+ cell lines can be
       efficiently propagated in vitro by repeated cycles of stimulation with
       tumor cells stably expressing B7.1. Professional APC and CD4+ helper
       cells are not required as far as interleukin 2 is exogenously provided.
       Furthermore, CD8+ blasts needed both signal 1 (Ag in the contest of the
       MHC molecule) and signal 2 (interaction of costimulatory molecules) for
       restimulation. T cell blasts in the presence of signal 1 or 2 only still
       retained their effector potential but did not undergo clonal expansion.
       These results are very promising for further applications of specific
       immunotherapies in humans.
 DE    Antigen Presentation/*IMMUNOLOGY  Antigens, CD80/*BIOSYNTHESIS/GENETICS
       Coculture  *Cytotoxicity, Immunologic  CD8-Positive
       T-Lymphocytes/*IMMUNOLOGY  DNA, Complementary/GENETICS  Gene Expression
       Human  Lymphocyte Transformation/IMMUNOLOGY  Lymphoma,
       T-Cell/GENETICS/*IMMUNOLOGY  Support, Non-U.S. Gov't  Tumor Cells,
       Cultured  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

