       Document 0733
 DOCN  M9640733
 TI    A manual bead assay for the determination of absolute CD4+ and CD8+
       lymphocyte counts in human immunodeficiency virus-infected individuals.
 DT    9604
 AU    Carella AV; Moss MW; Provost V; Quinn TC; Department of Medicine, Johns
       Hopkins University School of; Medicine, Baltimore, Maryland, USA.
 SO    Clin Diagn Lab Immunol. 1995 Sep;2(5):623-5. Unique Identifier :
       AIDSLINE MED/96089402
 AB    CD4+ T lymphocytes are currently the most common surrogate marker
       indicating disease progression in individuals infected with human
       immunodeficiency virus (HIV). Since the cost of enumerating lymphocyte
       phenotypes is quite high, an inexpensive bead assay analyzed by light
       microscopy (cytosphere assay; Coulter Corporation, Hialeah, Fla.) was
       developed as an alternative method for counting CD4+ and CD8+ T
       lymphocytes. To evaluate the reliability of the cytosphere assay,
       heparinized blood was collected from 117 HIV-infected individuals and
       tested for both CD4+ and CD8+ lymphocytes by flow cytometry and the
       cytosphere assay. The Pearson correlation coefficient of the cytosphere
       assay compared with that of flow cytometry for CD4+ T lymphocytes was
       0.93, with mean values +/- standard deviations of 534 +/- 509 by flow
       cytometry and 499 +/- 477 by the cytosphere assay. The correlation
       coefficient for CD8+ T lymphocytes was 0.86, with mean values of 831 +/-
       543 by flow cytometry and 746 +/- 472 by the cytosphere assay. The
       sensitivity and specificity of the cytosphere assay in determining
       absolute CD4+ T-lymphocyte counts of less than 200/microliters were 97.6
       and 94.7%, respectively. The positive predictive value was 90.9%, and
       the negative predictive value was 98.6%. The cytosphere assay was highly
       correlative to flow cytometry in determining CD4+ and CD8+ T-lymphocyte
       counts among HIV-infected patients. The ease and limited resources
       needed to perform this test make it ideal in developing countries and
       other areas where technology and finances are limited.
 DE    Cell Separation/*METHODS  Comparative Study  CD4 Lymphocyte
       Count/*METHODS  CD8-Positive T-Lymphocytes/*IMMUNOLOGY  Flow Cytometry
       Human  HIV Infections/*BLOOD/IMMUNOLOGY  Microspheres  Reproducibility
       of Results  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

