       Document 0743
 DOCN  M9640743
 TI    Removal of human immunodeficiency virus by an 0.04-micron membrane
       filter.
 DT    9604
 AU    Oshima KH; Comans TW; Highsmith AK; Ades EW; Biological Products Branch,
       Centers for Disease Control and; Prevention, Atlanta, Georgia 30333,
       USA.
 SO    J Acquir Immune Defic Syndr Hum Retrovirol. 1995 Jan 1;8(1):64-5. Unique
       Identifier : AIDSLINE MED/96142202
 AB    We tested the ability of a 0.04-micron nylon membrane filter to remove
       human immunodeficiency virus (HIV) from tissue culture media containing
       10% fetal calf serum. Endpoint titrations of infectious virus (ID50)
       were performed on lymphocyte cultures. The presence of virus in the
       cultures was determined using an enzyme-linked immunoabsorbant assay
       (ELISA) of the HIV-1 P24 core antigen. In repeated experiments,
       filtration of the virus suspension resulted in the removal of HIV below
       detectable limits. The titer reduction was estimated to be greater than
       8.5 x 10(2). These results suggest that this filter is effective in
       removing HIV from fluids containing serum or serum products.
 DE    Culture Media  Enzyme-Linked Immunosorbent Assay  Filtration/METHODS
       Human  HIV Core Protein p24/ANALYSIS  HIV-1/*ISOLATION & PURIF
       Lymphocytes/VIROLOGY  *Membranes, Artificial  *Micropore Filters  Nylons
       Support, Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.
       T-Lymphocytes/VIROLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

