       Document 0762
 DOCN  M9640762
 TI    Amino acid sequence requirements for the incorporation of the Vpx
       protein of simian immunodeficiency virus into virion particles.
 DT    9604
 AU    Park IW; Sodroski J; Division of Human Retrovirology, Dana-Farber Cancer
       Institute,; Boston, MA 02115, USA.
 SO    J Acquir Immune Defic Syndr Hum Retrovirol. 1995 Dec 15;10(5):506-10.
       Unique Identifier : AIDSLINE MED/96142212
 AB    To investigate the amino acid sequence determinants for the
       incorporation of Vpx protein into virion particles, several mutations
       were introduced into the vpx gene of SIVmac239 proviral DNA, and the
       effects of mutations on the expression and assembly of the Vpx protein
       were studied. The results show that deletions of amino acids from 78 to
       80 or from 82 to 87 abolished the incorporation of the expressed Vpx
       protein into virion particles. Other Vpx mutants, including a
       full-length Vpx-Vpr fusion protein and a mutant with a deletion of the
       C-terminal polyproline tract, were packaged efficiently. This study
       suggests that amino acids from 78 to 80 and 82 to 87 are important for
       the assembly of the Vpx protein into virus particles.
 DE    Amino Acid Sequence  DNA Mutational Analysis  DNA, Viral/GENETICS
       Electrophoresis, Polyacrylamide Gel  Gene Expression Regulation, Viral
       Gene Products, vpr/GENETICS/METABOLISM  Molecular Sequence Data
       Plasmids  Proviruses/GENETICS/METABOLISM  Sequence Deletion  Support,
       Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.
       SIV/GENETICS/*METABOLISM/PHYSIOLOGY  Transfection  Viral Regulatory
       Proteins/CHEMISTRY/GENETICS/*METABOLISM
       Virion/GENETICS/*METABOLISM/PHYSIOLOGY  Virus Replication  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

