       Document 0764
 DOCN  M9640764
 TI    HIV gp120-specific cell-mediated immune responses in mice after oral
       immunization with recombinant Salmonella.
 DT    9604
 AU    Berggren RE; Wunderlich A; Ziegler E; Schleicher M; Duke RC; Looney D;
       Fang FC; Division of Infectious Diseases, University of Colorado Health;
       Sciences Center, Denver 80267, USA.
 SO    J Acquir Immune Defic Syndr Hum Retrovirol. 1995 Dec 15;10(5):489-95.
       Unique Identifier : AIDSLINE MED/96142210
 AB    Salmonella is of great interest as a potential human immunodeficiency
       virus vaccine vector because of its ability to elicit potent mucosal and
       systemic immune responses when administered orally. To determine whether
       such a vaccine could elicit an immune response in mice, plasmids
       expressing HIV gp120-LAI were introduced into attenuated S. typhimurium.
       Three serial doses of 10(10) recombinant organisms were administered
       orally to BALB/c mice at 2-week intervals. Immunized mice but not
       control mice demonstrated proliferative T cell responses to gp120-LAI,
       comparable in magnitude to the proliferative responses to Salmonella
       antigens. Immunized mice had detectable serum and intestinal
       Salmonella-specific IgA and serum Salmonella-specific IgG. However, no
       gp120-specific antibody was detected in either serum or intestinal
       washes. These results indicate that live recombinant Salmonella-based
       vaccine constructs can induce HIV-specific cellular immune responses in
       vivo.
 DE    Administration, Oral  Animal  Antibodies, Bacterial/ANALYSIS  AIDS
       Vaccines/ADMINISTRATION & DOSAGE/*IMMUNOLOGY  Base Sequence  DNA
       Primers/CHEMISTRY  Enzyme-Linked Immunosorbent Assay  Female  HIV
       Antibodies/ANALYSIS  HIV Envelope Protein gp120/*IMMUNOLOGY  HIV
       Infections/*IMMUNOLOGY  HIV-1/*IMMUNOLOGY  Immunity, Cellular
       *Immunization  Intestinal Mucosa/IMMUNOLOGY  Lymphocyte
       Transformation/IMMUNOLOGY  Male  Mice  Mice, Inbred BALB C  Molecular
       Sequence Data  Plasmids  Salmonella typhimurium/*IMMUNOLOGY  Support,
       Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.  T-Lymphocytes/IMMUNOLOGY
       Vaccines, Synthetic/ADMINISTRATION & DOSAGE/*IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

