       Document 0853
 DOCN  M9640853
 TI    Diversity of immunobiological functions of T-cell lines established from
       patients with adult T-cell leukaemia.
 DT    9604
 AU    Iwatsuki K; Harada H; Motoki Y; Kaneko F; Jin F; Takigawa M; Department
       of Dermatology, Fukushima Medical College, Japan.
 SO    Br J Dermatol. 1995 Dec;133(6):861-7. Unique Identifier : AIDSLINE
       MED/96150394
 AB    In order to understand the variety of HTLV-1-associated cutaneous
       diseases, we studied the cytological profile of HTLV-1-infected T-cell
       lines established from patients with adult T-cell leukaemia (ATL). Among
       four CD4+ cell lines, termed 16T(-), 35T(-), MH-1, and KS-2, the 16T(-)
       cells secreted elevated quantities of IL-4, IL-6 and IFN-gamma and
       expressed mRNA for each cytokine in the absence of exogenous
       stimulation. The 35T(-) cells secreted IL-6 and a small amount of
       IFN-gamma, but not IL-4. The MH-1 and KS-2 cells secreted only IL-6 in
       the absence of stimulation. In response to stimulation with
       phorbol-12-myristate-13 acetate (PMA), the 16T(-) cells produced more
       IL-4 and IFN-gamma, whereas the 35T(-) and MH-1 cells exhibited
       increased secretion of IFN-gamma, but still no IL-4 or IL-4 mRNA
       production. Although neither IL-4 nor IFN-gamma were found in the
       culture supernatant of KS-2 cells, the production of IL-4 mRNA was
       detected by RT-PCR. Culture supernatants from the 16T(-) and 35T(-)
       cells induced the expression of intercellular adhesion molecule-1
       (ICAM-1) and HLA-DR by cultured keratinocytes. This response was
       inhibited by pretreatment of the supernatant with anti-IFN-gamma
       antibodies. These results indicate that some HTLV-1-infected T-cell
       lines constitutively secrete various cytokines, including biologically
       active IFN-gamma. The diversity of immunobiological functions of the
       T-cell lines may be related to the variety of clinical features present
       in ATL patients.
 DE    Adult  Antigens, Viral/ANALYSIS  Base Sequence  Cell Line  Culture
       Media, Conditioned/PHARMACOLOGY  Cytokines/ANALYSIS  DNA
       Primers/GENETICS  DNA, Viral/ANALYSIS  Human  HLA-DR Antigens/METABOLISM
       *HTLV-I/GENETICS/IMMUNOLOGY  Intercellular Adhesion
       Molecule-1/METABOLISM  Keratinocytes/METABOLISM  Leukemia,
       T-Cell/*IMMUNOLOGY/VIROLOGY  Molecular Sequence Data  Polymerase Chain
       Reaction  RNA, Messenger/ANALYSIS  T-Lymphocytes/*IMMUNOLOGY  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

