       Document 0858
 DOCN  M9640858
 TI    Loss of Gag-specific antibody reactivity in cattle experimentally
       infected with bovine immunodeficiency-like virus.
 DT    9604
 AU    Isaacson JA; Roth JA; Wood C; Carpenter S; Department of Microbiology,
       Immunology and Preventive Medicine,; College of Veterinary Medicine,
       Iowa State University, Ames; 50011, USA.
 SO    Viral Immunol. 1995;8(1):27-36. Unique Identifier : AIDSLINE
       MED/96124511
 AB    The development and persistence of virus-specific antibodies were
       investigated in eight cattle experimentally infected with the R29
       isolate of bovine immunodeficiency-like virus (BIV). By 4 weeks
       postinoculation (p.i.), antibodies reactive to BIV gag- and env-encoded
       recombinant fusion proteins were detectable by immunoblotting in all
       animals. By 40 weeks p.i., seven of eight cattle had dramatically
       decreased Gag-specific antibodies, and anti-Gag reactivity remained very
       low or undetectable through 190 weeks p.i. Immunoprecipitation
       experiments revealed a similar loss of reactivity to nondenatured BIV
       Gag in these animals. In contrast, antibodies to a recombinant BIV Env
       protein were readily detectable throughout the study in all eight
       cattle. During the period of declining Gag antibody, infectious virus
       was recoverable from peripheral blood mononuclear cells of each animal.
       However, there was no evidence for sufficient amounts of BIV
       p26-containing immune complexes to explain the loss of anti-Gag
       reactivity. Interestingly, the single animal that maintained detectable
       anti-Gag reactivity throughout the study was repeatedly negative for
       virus recovery beyond 17 weeks p.i. All animals have remained clinically
       normal for over 4 years p.i., with no evidence of consistent changes in
       mononuclear cell subsets. These findings provide evidence that in BIV
       infection an early decline in Gag-specific antibody reactivity can occur
       without evidence of increasing viral replication or progression to overt
       clinical disease.
 DE    Animal  Antibodies, Viral/*CHEMISTRY  Antibody Specificity  Cattle
       Cattle Diseases/*IMMUNOLOGY/VIROLOGY  Gene Products, gag/*IMMUNOLOGY
       Immunoblotting  Immunodeficiency Virus, Bovine/*IMMUNOLOGY/ISOLATION &
       PURIF  Lentivirus Infections/*IMMUNOLOGY/*VETERINARY  Male  Precipitin
       Tests  Recombinant Proteins/IMMUNOLOGY  Support, U.S. Gov't, P.H.S.
       Viral Envelope Proteins/IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

