       Document 0098
 DOCN  M9650098
 TI    Differential effects of gonadectomy on thymic stromal cells in promoting
       T cell differentiation in mice.
 DT    9605
 AU    Utsuyama M; Hirokawa K; Mancini C; Brunelli R; Leter G; Doria G;
       Department of Immunopathology, Tokyo Metropolitan Institute of;
       Gerontology, Japan.
 SO    Mech Ageing Dev. 1995 Jul 14;81(2-3):107-17. Unique Identifier :
       AIDSLINE MED/96171086
 AB    Twenty-six week-old BDF1 mice were gonadectomized and grafted with
       thymus from irradiated (8.5 Gy) newborn, 6-week-old, or 26-week-old
       mice. One month later, grafted thymuses were recovered and examined in
       terms of thymocyte numbers, subpopulations and proliferative responses
       to Concananavlin A (Con A). The growth of the irradiated thymus was
       significantly higher in gonadectomized (Gx) than in sham-operated (Sham)
       mice and the magnitude of thymic growth was apparently age-dependent, as
       it was greater for newborns than for older mice. Con A response of
       thymocytes was also significantly higher in Gx mice than in Sham mice,
       and the magnitude of the response declined with advancing age of the
       thymus donors. Flow cytometric analysis revealed that a significant
       increase in the percentage of CD4+CD8- was observed in thymus grafts
       showing high Con A responses. However, this effect of Gx on the thymus
       graft was dependent on age of the thymus donor. Namely, newborn thymus
       grafts could grow equally well in both Gx and Sham recipients, whereas
       thymus grafts from 6- and 26-week-old mice could grow well only in Gx,
       but not in Sham recipients. The number of thymocytes was comparable in
       thymus grafts from 6- and 26-week-old mice, but the proliferative
       response to Con A was higher in the former than in the latter graft.
       Collectively, Gx appeared to promote immigration of thymocyte precursors
       into the thymus and to enhance proliferation and differentiation of
       thymocytes towards CD4+CD8- T cells, in an age-related manner.
 DE    Aging/*PATHOLOGY  Animal  Animals, Newborn  Cell
       Differentiation/PHYSIOLOGY  Comparative Study  CD4-CD8 Ratio  Evaluation
       Studies  Male  Mice  Mice, Inbred C57BL  Mice, Inbred DBA  Orchiectomy
       Stromal Cells/PHYSIOLOGY  T-Lymphocytes/*CYTOLOGY  Testis/*PHYSIOLOGY
       Thymus Gland/TRANSPLANTATION  Thymus Hyperplasia/*PATHOLOGY  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

