       Document 0153
 DOCN  M9650153
 TI    Dynamics of viral replication in infants with vertically acquired human
       immunodeficiency virus type 1 infection.
 DT    9605
 AU    De Rossi A; Masiero S; Giaquinto C; Ruga E; Comar M; Giacca M;
       Chieco-Bianchi L; Institute of Oncology, InterUniversity Center for
       Cancer; Research, AIDS Reference Center, University of Padova, Italy.
 SO    J Clin Invest. 1996 Jan 15;97(2):323-30. Unique Identifier : AIDSLINE
       MED/96149157
 AB    About one-third of vertically HIV-1 infected infants develop AIDS within
       the first months of life; the remainder show slower disease progression.
       We investigated the relationship between the pattern of HIV-1
       replication early in life and disease outcome in eleven infected infants
       sequentially studied from birth. Viral load in cells and plasma was
       measured by highly sensitive competitive PCR-based methods. Although all
       infants showed an increase in the indices of viral replication within
       their first weeks of life, three distinct patterns emerged: (a) a rapid
       increase in plasma viral RNA and cell-associated proviral DNA during the
       first 4-6 wk, reaching high steady state levels (> 1,000 HIV-1
       copies/10(5) PBMC and > 1,000,000 RNA copies/ml plasma) within 2-3 mo of
       age; (b) a similar initial rapid increase in viral load, followed by a
       2.5-50-fold decline in viral levels; (c) a significantly lower (>
       10-fold) viral increase during the first 4-6 wk of age. All infants
       displaying the first pattern developed early AIDS, while infants with
       slower clinical progression exhibited the second or third pattern. These
       findings demonstrate that the pattern of viral replication and clearance
       in the first 2-3 mo of life is strictly correlated with, and predictive
       of disease evolution in vertically infected infants.
 DE    Disease Transmission, Vertical  DNA, Viral/ANALYSIS  Human  HIV
       Infections/*MICROBIOLOGY  HIV-1/*GROWTH & DEVELOPMENT/GENETICS  Infant
       Infant, Newborn  Polymerase Chain Reaction/METHODS  Proviruses/CHEMISTRY
       RNA, Viral/ANALYSIS  Support, Non-U.S. Gov't  Virion/CHEMISTRY  Virus
       Replication  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

