       Document 0255
 DOCN  M9650255
 TI    Characterization of Encephalitozoon (Septata) intestinalis isolates
       cultured from nasal mucosa and bronchoalveolar lavage fluids of two AIDS
       patients.
 DT    9605
 AU    Didier ES; Rogers LB; Orenstein JM; Baker MD; Vossbrinck CR; Van Gool T;
       Hartskeerl R; Soave R; Beaudet LM; Department of Microbiology, Tulane
       Regional Primate Research; Center, Covington, Louisiana 70433, USA.
 SO    J Eukaryot Microbiol. 1996 Jan-Feb;43(1):34-43. Unique Identifier :
       AIDSLINE MED/96151459
 AB    Microsporidia are obligate intracellular protozoan parasites that can
       cause opportunistic infections in AIDS patients. Species from five
       genera of microsporidia are presently known to infect man. One species,
       Septata intestinalis originally was detected in stool specimens of
       individuals with chronic diarrhea and subsequently was found to
       disseminate to the kidneys, lungs, and nasal sinuses. This organism has
       since been reclassified as Encephalitozoon and in this study, we report
       the culture of Encephalitozoon intestinalis from a bronchoalveolar
       lavage specimen and a nasal mucus aspirate of two AIDS patients living
       in the USA. The bronchoalveolar and nasal microsporidian isolates grew
       in several continuous cell lines including RK-13, MDCK, HT-29, Caco-2,
       Vero, and I047. Transmission electron microscopy of the clinical and
       cell culture specimens revealed that the new isolates appeared to be E.
       intestinalis based on morphology and growth of organisms in septated
       membrane-bound parasitophorous vacuoles. The new E. intestinalis
       isolates were characterized and compared with the first isolated E.
       intestinalis that was cultured from stool to confirm their identity and
       to determine if there existed any minor differences, as seen in the
       closely related Encephalitozoon cuniculi strains. By the methods of
       sodium dodecyl sulfate-polyacrylamide gel electrophoresis staining for
       proteins and carbohydrates, Western blot immunodetection, and polymerase
       chain reaction-based methods with restriction endonuclease digestion,
       double-stranded DNA heteroduplex mobility shift analysis, and DNA
       sequencing of the ribosomal DNA intergenic spacer region, the new
       isolates were identical to each other and to the reference isolate of E.
       intestinalis. In addition, with any of these methods, the E.
       intestinalis organisms could be distinguished from the three E. cuniculi
       strains, Encephalitozoon hellem, and Vittaforma corneae, which is
       important for diagnostics, therapeutic strategies, and epidemiology.
 DE    Acquired Immunodeficiency Syndrome/COMPLICATIONS/*PARASITOLOGY  Animal
       AIDS-Related Opportunistic Infections/*PARASITOLOGY  Base Sequence
       Bronchoalveolar Lavage Fluid/*PARASITOLOGY  DNA, Ribosomal/GENETICS
       Encephalitozoon/GENETICS/IMMUNOLOGY/*ISOLATION & PURIF/  ULTRASTRUCTURE
       Encephalitozoonosis/COMPLICATIONS  Glycoproteins/ISOLATION & PURIF
       Human  Molecular Sequence Data  Nasal Mucosa/*PARASITOLOGY  Protozoan
       Proteins/ISOLATION & PURIF  Sequence Analysis, DNA  Support, Non-U.S.
       Gov't  Support, U.S. Gov't, P.H.S.  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

