       Document 0084
 DOCN  M9490084
 TI    The majority of neutralizing Abs in HIV-1-infected patients recognize
       linear V3 loop sequences. Studies using HIV-1MN multiple antigenic
       peptides.
 DT    9411
 AU    Vogel T; Kurth R; Norley S; Paul Ehrlich Institute, Langen, Germany.
 SO    J Immunol. 1994 Aug 15;153(4):1895-904. Unique Identifier : AIDSLINE
       MED/94321799
 AB    Multiple antigenic peptides (MAP) comprising eight synthetic peptides
       corresponding to the V3 loop of HIV-1MN or HIV-1IIIB linked to a lysine
       core were used to characterize the humoral Ab response of HIV-1-infected
       patients against this domain. One hundred percent of the tested sera
       from HIV-1-infected European patients reacted significantly with the
       HIV-1MN V3 loop MAP. Most, but not all, sera also reacted against the
       HIV-1IIIB V3 loop MAP, albeit with low titers. The in vitro
       neutralization capacity of the patients' sera against HIV-1MN or
       HIV-1IIIB infection were tested. All sera showed significant
       neutralizing titers against HIV-1MN, in comparison with the low (when
       detectable) neutralizing titers against HIV-1IIIB. Competition assays
       revealed that the majority of HIV-1MN-neutralizing Abs in patients' sera
       react with the V3 loop peptides. Only a small fraction of the total
       neutralizing capacity, presumably specific for other neutralizing
       epitopes, could not be adsorbed with HIV-1MN V3 loop peptides. The V3
       loop peptides could also compete for the binding of Abs in patients'
       sera mediating Ab-dependent cellular cytotoxicity (ADCC) of HIV-1MN
       infected cells, inhibiting 30 to 80% of the ADCC activity. Finally, the
       HIV-1MN V3 loop MAP, when used as immunogen in the presence of RIBI
       adjuvant without a carrier protein, elicited Abs able to neutralize
       HIV-1MN (but not HIV-1IIIB) and to initiate ADCC of HIV-1MN-infected
       cells. This neutralizing and ADCC activity, mediated by V3MN-specific
       rabbit Abs, could be totally absorbed by V3MN loop peptides.
 DE    Amino Acid Sequence  Antibody Specificity  Antibody-Dependent Cell
       Cytotoxicity/IMMUNOLOGY  Antigenic Determinants  Human  HIV
       Antibodies/*IMMUNOLOGY  HIV Envelope Protein gp120/*IMMUNOLOGY  HIV
       Infections/*IMMUNOLOGY  HIV-1/*IMMUNOLOGY  Molecular Sequence Data
       Neutralization Tests  Peptide Fragments/*IMMUNOLOGY
       Peptides/CHEMISTRY/IMMUNOLOGY  Support, Non-U.S. Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

