       Document 0124
 DOCN  M9490124
 TI    Clinical importance of extraordinary integration patterns of human
       T-cell lymphotropic virus type I proviral DNA in adult T-cell
       leukemia/lymphoma.
 DT    9411
 AU    Shimamoto Y; Suga K; Shibata K; Matsuzaki M; Yano H; Yamaguchi M;
       Department of Internal Medicine, Saga Medical School, Japan.
 SO    Blood. 1994 Aug 1;84(3):853-8. Unique Identifier : AIDSLINE MED/94318962
 AB    The proviral DNA of human T-cell lymphotrophic virus type I (HTLV-I) is
       known to be integrated monoclonally in the malignant cells of adult
       T-cell leukemia/lymphoma (ATL), which is a peripheral T-cell malignancy
       caused by this virus. We studied the relationship between the
       integration patterns of HTLV-I and clinical characteristics in 89
       patients with ATL. The proviral DNA of HTLV-I was examined by the
       standard Southern blot analysis using the endonucleases EcoRI and Pst I.
       One clear band of greater than 9 kb was detected in most of the patients
       (83 case) when cellular DNA was digested with EcoRI. On the other hand,
       extraordinary integration patterns of HTLV-I proviral DNA were detected
       in 6 patients; 3 of them showed two bands, while the other 3 showed one
       band smaller than 9 kb. When cellular DNA was digested with PstI, the
       band patterns of these patients were quite different from those of
       typical patients. The patients with the extraordinary integration
       patterns had clinical characteristics dissimilar to those of the other
       83 patients with the ordinary integration pattern. The patients with two
       bands by EcoRI digestion always had severe hypoxemia with extremely high
       levels of serum lactate dehydrogenase at first presentation and showed
       peculiar organ infiltrations, such as retina and muscle, which were less
       frequent in the other ordinary 83 patients. They all died within 8
       months after the onset. In contrast, the patients with one smaller band
       by EcoRI digestion always had small and mature T lymphocytes with
       bilobulated nuclei without lymphadenopathy and showed a favorable
       clinical course, which was uncommon in the ordinary cases. They were
       alive 20 to 38 months after diagnosis. Rearranged bands of the T-cell
       receptor gene were detected in all patients with unusual integration.
       These findings indicate that the integration patterns of HTLV-I proviral
       DNA have a clinical implication and may be one of the explanations for
       heterogeneity in the behavior of this disease.
 DE    DNA, Viral/*GENETICS/METABOLISM  Female  Gene Rearrangement,
       B-Lymphocyte  Human  HTLV-I/*GENETICS  Leukemia, T-Cell/*MICROBIOLOGY
       Male  Proviruses  Restriction Mapping  Support, Non-U.S. Gov't  Virus
       Integration  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

