       Document 0403
 DOCN  M9490403
 TI    Identification of an H-2 Kb-presented Moloney murine leukemia virus
       cytotoxic T-lymphocyte epitope that displays enhanced recognition in H-2
       Db mutant bm13 mice.
 DT    9411
 AU    Sijts AJ; De Bruijn ML; Ressing ME; Nieland JD; Mengede EA; Boog CJ;
       Ossendorp F; Kast WM; Melief CJ; Department of Immunohematology and
       Blood Bank, University; Hospital Leiden, The Netherlands.
 SO    J Virol. 1994 Sep;68(9):6038-46. Unique Identifier : AIDSLINE
       MED/94335121
 AB    Upon infection with the Moloney murine sarcoma virus-murine leukemia
       virus (MuLV) complex, H-2b C57BL/6 (B6) mice respond with a class I
       Db-restricted cytotoxic T-lymphocyte (CTL) response, which protects
       against virus-induced tumorigenesis. In the B6-derived Db mutant
       B6.CH-2bm13 (bm13) strain, part of the class I Db antigen-presenting
       groove is shaped by a class I Kb-encoded sequence. Like B6 mice, bm13
       mice reject Moloney virus-induced tumors, but the protective CTL
       response is Kb restricted. In this study we show enhanced levels of
       Moloney MuLV-specific CTLp with a restriction for Kb in bm13 mice.
       Through the use of CTL clones from Moloney virus-immunized bm13 mice,
       the class I Kb-presented CTL epitope was identified. The epitope is
       located in the Moloney virus gp70 envelope protein region (Moloney
       envelope, amino acids 189 to 196 [Mol env (189-196)]), SSWDFITV and has
       the Kb allele-specific binding motif. The Dbm13 molecule does not
       present the env(189 to 196) epitope to Kb-restricted bm13 CTL. In B6
       mice, Mol env(189-196)-specific CTL could be induced by peptide
       vaccination. B6 mice thus have CTL precursors specific for this epitope
       but at considerably lower levels than do bm13 mice. We hypothesize that
       additional positive selection of Kb-restricted CTL on the Dbm13 molecule
       in bm13 mice explains this difference in precursor frequencies. We
       examined related strains of MuLV for the presence of Mol env(189-196)
       sequence equivalents. Rauscher, Friend, and AKV MuLV-encoded Mol
       env(189-196) epitope equivalents were properly recognized in
       cytotoxicity assays, both as synthetic and as endogenously expressed
       (Rauscher MuLV) peptides. In contrast, the mink cell focus-forming virus
       MuLV-encoded epitope equivalent, lacking a Kb anchor residue, was not
       presented for CTL recognition and hence can be excluded as an important
       CTL epitope for mink cell focus-forming viruses.
 DE    Amino Acid Sequence  Animal  Antigen-Presenting Cells/IMMUNOLOGY
       Antigenic Determinants  Antigens, Viral/*IMMUNOLOGY  Gene Products,
       env/IMMUNOLOGY  H-2 Antigens/*IMMUNOLOGY/METABOLISM  Mice  Mice, Inbred
       C57BL  Mice, Mutant Strains  Molecular Sequence Data  Moloney Leukemia
       Virus/*IMMUNOLOGY  Peptides/IMMUNOLOGY  Support, Non-U.S. Gov't
       T-Lymphocytes, Cytotoxic/*IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

