       Document 0281
 DOCN  M94A0281
 TI    Comparison of nested PCR for detection of DNA in plasma with pp65
       leukocytic antigenemia procedure for diagnosis of human cytomegalovirus
       infection.
 DT    9412
 AU    Freymuth F; Gennetay E; Petitjean J; Eugene G; Hurault de Ligny B;
       Ryckelynck JP; Legoff C; Hazera P; Bazin C; Virus Laboratory, University
       Hospital Caen, France.
 SO    J Clin Microbiol. 1994 Jun;32(6):1614-8. Unique Identifier : AIDSLINE
       MED/94358148
 AB    A nested PCR was used for the detection of human cytomegalovirus (HCMV)
       DNA in plasma. The presence of HCMV DNA and its correlation to pp65
       leukocytic antigenemia were investigated with 299 blood samples from 45
       organ transplant recipients and 63 AIDS patients. Of the 53 samples
       positive by nested PCR, 52 (98%) were also positive for leukocytic
       antigenemia and 23 had high levels of antigenemia (> 50 positive cells
       per 2 x 10(5) leukocytes). Of the 246 samples negative in PCR, only 3
       (1.2%) had highly positive antigenemia. For 15 patients having a high
       antigenemia level in the course of their disease, consecutive blood
       samples were studied and also assessed for viremia in culture. The
       extent to which HCMV DNA, detected by PCR, was present in plasma
       correlated with increased levels of HCMV leukocytic antigenemia for six
       of the eight AIDS patients and for all the organ transplant recipients.
       Positivity for HCMV DNA in PCR and for viremia in cell culture was
       usually restricted to the highest antigenemia levels. From a total of 69
       blood samples, PCR and culture gave positive results, respectively, for
       17 of 32 samples (53%) and 14 of 32 samples (43%) from transplant
       recipients and for 15 of 37 samples (40%) and 9 of 37 samples (24%) from
       AIDS patients. Our findings have shown a strong correlation between high
       levels of leukocytic antigenemia and HCMV DNA in plasma. The detection
       of HCMV DNA in plasma by this nested PCR can prove HCMV dissemination in
       blood, but it lacks the rapidity and simplicity of the leukocytic pp65
       antigenemia procedure.
 DE    AIDS-Related Opportunistic Infections/MICROBIOLOGY  Base Sequence
       Comparative Study  Cytomegalovirus/GENETICS/IMMUNOLOGY/*ISOLATION &
       PURIF  Cytomegalovirus Infections/*MICROBIOLOGY  DNA Primers  DNA,
       Viral/*BLOOD  Human  Leukocytes/*MICROBIOLOGY  Molecular Sequence Data
       Phosphoproteins/*BLOOD  *Polymerase Chain Reaction  Postoperative
       Complications/MICROBIOLOGY  Support, Non-U.S. Gov't  Transplantation
       Viral Matrix Proteins/*BLOOD  Viremia/*MICROBIOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

