       Document 0282
 DOCN  M94A0282
 TI    Comparison of techniques and evaluation of three commercial monoclonal
       antibodies for laboratory diagnosis of varicella-zoster virus in
       mucocutaneous specimens.
 DT    9412
 AU    Perez JL; Garcia A; Niubo J; Salva J; Podzamczer D; Martin R; Service of
       Microbiology, Hospital Princeps d'Espanya, Ciutat; Sanitaria i
       Universitaria de Bellvitge, L'Hospitalet de; Llobregat, Barcelona,
       Spain.
 SO    J Clin Microbiol. 1994 Jun;32(6):1610-3. Unique Identifier : AIDSLINE
       MED/94358147
 AB    A comparison of direct antigen detection in cell scrapings with culture
       techniques (tube culture and shell vial method) for diagnosis of
       varicella-zoster virus (VZV) mucocutaneous infections was done in
       parallel in two groups of specimens. A total of 100 specimens were from
       patients with clinical diagnosis of VZV infection (group 1), and 69 were
       from patients with no suspicion of VZV infection (group 2) but mainly
       with herpes simplex virus infections. In addition, three commercially
       available monoclonal antibodies (Whittaker, Biosoft Clone 2013, and
       Ortho 3B3) directed against VZV antigens were evaluated in parallel in
       the last 87 group 1 specimens. Overall, 80% of the group 1 specimens
       were confirmed positive by direct detection, in comparison with 56%
       positive by tube culture and/or shell vial. None of the group 2
       specimens were positive for VZV by any of the methods, and none of the
       monoclonal antibodies assayed reacted with any herpes simplex virus
       stock strains. Antiviral therapy and the length of evolution time of
       lesions affected negatively the performance of all laboratory methods,
       but to a lesser extent in direct detection techniques than in culture
       techniques. The Whittaker and Biosoft reagents (indirect
       immunofluorescence assay) showed statistically significant differences
       in sensitivity with respect to the Ortho antibody (P = 0.002 and P =
       0.039, respectively; two-tailed binomial test). Direct antigen detection
       is a rapid, easy-to-perform, sensitive, and specific technique and
       appears to be the method of choice for laboratory confirmation of VZV
       mucocutaneous infections.
 DE    Antibodies, Monoclonal/*IMMUNOLOGY  Antibodies, Viral/*IMMUNOLOGY
       Antigens, Viral/*ANALYSIS  Antiviral Agents/THERAPEUTIC USE
       AIDS-Related Opportunistic Infections/DIAGNOSIS/MICROBIOLOGY
       Comparative Study  Cross Reactions  Evaluation Studies  Herpes
       Zoster/COMPLICATIONS/*DIAGNOSIS/DRUG THERAPY  Herpesvirus 3,
       Human/GROWTH & DEVELOPMENT/IMMUNOLOGY/*ISOLATION &  PURIF  Human  Mucous
       Membrane/*MICROBIOLOGY  Neoplasms/COMPLICATIONS  Postoperative
       Complications/MICROBIOLOGY  Skin/*MICROBIOLOGY  Virus Cultivation
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

