       Document 0356
 DOCN  M94A0356
 TI    TaqStart Antibody: hot start PCR facilitated by a neutralizing
       monoclonal antibody directed against Taq DNA polymerase.
 DT    9412
 AU    Kellogg DE; Rybalkin I; Chen S; Mukhamedova N; Vlasik T; Siebert PD;
       Chenchik A; CLONTECH Laboratories, Palo Alto, CA.
 SO    Biotechniques. 1994 Jun;16(6):1134-7. Unique Identifier : AIDSLINE
       MED/94355058
 AB    The specificity and DNA yield of PCRs are often improved by the hot
       start technique and analogous methods. The intent of the approach is to
       eliminate or prevent the generation of nonspecific PCR templates that
       may be synthesized at ambient temperature prior to thermal cycling.
       Monoclonal antibodies (MAbs) raised in mice to the purified DNA
       polymerase of Thermus aquaticus (Taq) were selected for their ability to
       reversibly block polymerase activity. The MAbs, incubated with Taq DNA
       polymerase and added to PCR tubes at ambient temperature, yield specific
       DNA fragments upon amplification when using high numbers of temperature
       cycles and a very low copy number of target DNA in a complex DNA
       background. This approach, using the TaqStart Antibody, permits the
       preparation of reaction mixtures at ambient temperatures without the
       subsequent opening of reaction tubes, use of grease or waxes, or of
       degradative enzymes and deoxyribonucleotide analogs.
 DE    Antibodies, Monoclonal/*PHARMACOLOGY  Base Sequence  DNA
       Polymerases/*ANTAGONISTS & INHIB/IMMUNOLOGY  DNA, Viral/ANALYSIS
       HIV/GENETICS  Molecular Sequence Data  Polymerase Chain
       Reaction/*METHODS  TECHNICAL REPORT  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

