       Document 2770
 DOCN  M94A2770
 TI    Saliva levels of HIV-1 antibodies during seroconversion.
 DT    9412
 AU    Sangare KA; Hofman L; Institute Pasteur de Cote D'Ivoire.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):229 (abstract no. PB0345). Unique
       Identifier : AIDSLINE ICA10/94369805
 AB    OBJECTIVE: In previous studies we, like many others, have shown that HIV
       antibodies can be detected in saliva. However, since the IgG level in
       saliva is approximately 400-1000 times less than that in serum, there
       has been concern that HIV antibodies will not be detected in saliva as
       soon as in serum. This study was undertaken to determine saliva levels
       in HIV positive individuals during seroconversion. METHODS: From a
       population at risk for HIV infection, 250 consenting adults were
       determined to be negative by the following assays: Abbott Recombinant
       HIV-1/HIV-2, 3rd generation; Enzygnost anti HIV-1/HIV-2; Vironostika
       Mixt. All subjects were free to leave the study at any time. Once a week
       saliva was collected from each subject with the Omni-SAL collector
       (Saliva Diagnostic Systems, Inc.). The specimen was tested with Abbott
       Test-PACK and Wellcozyme GACELISA on the day of collection. In addition
       a dried blood spot was collected and assayed according to the procedure
       in the Wellcozyme GACELISA protocol. If a positive was obtained with any
       test, a blood sample was taken the same day or as soon as possible for a
       Western Blot analysis. RESULTS: One individual had confirmed positive
       saliva and dried blood spot specimens in the third week of the study.
       Western Blots, run on serum samples obtained the next day and in the
       following weeks for up to two months, showed a progressive increase in
       the intensity and number of bands, starting with only p24 and gp160 and
       by two months showing, in addition p17, p31, gp41, p51, p55, p66 and
       gp120. No individuals had confirmed positive dried blood spot specimens
       that were not positive by saliva. CONCLUSION: Our study shows that
       saliva is as effective as a dried blood spot specimen in determining HIV
       positivity in early seroconversion.
 DE    Adult  Blotting, Western  Cohort Studies  Comparative Study
       Enzyme-Linked Immunosorbent Assay  Follow-Up Studies  Human  HIV
       Antibodies/*ANALYSIS/BLOOD  HIV Seropositivity/*DIAGNOSIS
       HIV-1/*IMMUNOLOGY  Reagent Kits, Diagnostic  Risk Factors
       Saliva/*IMMUNOLOGY  Salivary Proteins/*ANALYSIS  Specimen
       Handling/INSTRUMENTATION  DUPLICATE PUBLICATION  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

