       Document 0059
 DOCN  M9550059
 TI    Cloning and characterization of a c-myc intron binding protein (MIBP1).
 DT    9505
 AU    Makino R; Akiyama K; Yasuda J; Mashiyama S; Honda S; Sekiya T; Hayashi
       K; Oncogene Division, National Cancer Center Reseach Institute,; Tokyo,
       Japan.
 SO    Nucleic Acids Res. 1994 Dec 25;22(25):5679-85. Unique Identifier :
       AIDSLINE GENBANK/D37951
 AB    The cDNA for a c-myc intron 1 binding protein 1 (MIBP1) in the rat was
       isolated from lambda gt11 and lambda ZAPII cDNA libraries. Sequencing of
       the cDNA clones revealed a long ORF which encoded a putative protein of
       2437 amino acid residues. This protein has two widely separated zinc
       finger regions, each of which carries C2H2 motifs. When expressed in E.
       coli as a fusion protein, part of the MIBP1 showed sequence-specific
       binding to the target sequence, i.e., a 9-bp sequence in the rat c-myc
       intron 1. MIBP1 is most likely the rat counterpart of human MHC binding
       protein-2 (MBP-2/HIV-EP2), based on the 86% similarity in nucleotide
       sequence and 93% similarity in amno acid sequence. Northern blotting
       revealed a high level of MIBP1 mRNA in the brain.
 DE    Amino Acid Sequence  Animal  Base Sequence  Cloning, Molecular
       DNA-Binding Proteins/*GENETICS/METABOLISM  Gene Expression  *Genes, myc
       Introns  Molecular Sequence Data  Rats  RNA, Messenger/GENETICS
       Support, Non-U.S. Gov't  Transcription Factors/*GENETICS  Zinc Fingers
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

