       Document 0160
 DOCN  M9550160
 TI    Uses of flow cytometry in virology.
 DT    9505
 AU    McSharry JJ; Department of Microbiology, Immunology and Molecular
       Genetics,; Albany Medical College, New York 12208.
 SO    Clin Microbiol Rev. 1994 Oct;7(4):576-604. Unique Identifier : AIDSLINE
       MED/95136209
 AB    This article reviews some of the published applications of flow
       cytometry for in vitro and in vivo detection and enumeration of
       virus-infected cells. Sample preparation, fixation, and permeabilization
       techniques for a number of virus-cell systems are evaluated. The use of
       flow cytometry for multiparameter analysis of virus-cell interactions
       for simian virus 40, herpes simplex viruses, human cytomegalovirus, and
       human immunodeficiency virus and its use for determining the effect of
       antiviral compounds on these virus-infected cells are reviewed. This is
       followed by a brief description of the use of flow cytometry for the
       analysis of several virus-infected cell systems, including blue tongue
       virus, hepatitis C virus, avian reticuloendotheliosis virus, African
       swine fever virus, woodchuck hepatitis virus, bovine viral diarrhea
       virus, feline leukemia virus, Epstein-Barr virus, Autographa californica
       nuclear polyhedrosis virus, and Friend murine leukemia virus. Finally,
       the use of flow cytometry for the rapid diagnosis of human
       cytomegalovirus and human immunodeficiency virus in peripheral blood
       cells of acutely infected patients and the use of this technology to
       monitor patients on antiviral therapy are reviewed. Future prospects for
       the rapid diagnosis of in vivo viral and bacterial infections by flow
       cytometry are discussed.
 DE    Antibodies, Viral/ANALYSIS/ISOLATION & PURIF  Antigens, Viral/ISOLATION
       & PURIF  *Flow Cytometry  Forecasting  Human  Specimen Handling  Stains
       and Staining  Support, U.S. Gov't, P.H.S.  Virology/METHODS
       Viruses/IMMUNOLOGY/*ISOLATION & PURIF/*PHYSIOLOGY  JOURNAL ARTICLE
       REVIEW  REVIEW, TUTORIAL

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

