       Document 0321
 DOCN  M9550321
 TI    Three populations of cells with dendritic morphology exist in peripheral
       blood, only one of which is infectable with human immunodeficiency virus
       type 1.
 DT    9505
 AU    Weissman D; Li Y; Ananworanich J; Zhou LJ; Adelsberger J; Tedder TF;
       Baseler M; Fauci AS; Laboratory of Immunoregulation, National Institute
       of Allergy and; Infectious Diseases, National Institutes of Health,
       Bethesda, MD; 20892.
 SO    Proc Natl Acad Sci U S A. 1995 Jan 31;92(3):826-30. Unique Identifier :
       AIDSLINE MED/95148630
 AB    Conflicting data have been reported with regard to the infectability,
       dysfunction, and depletion of dendritic cells (DCs) in human
       immunodeficiency virus (HIV) disease. These discrepancies could
       potentially be explained by the existence of multiple subsets of cells
       with dendritic morphology in peripheral blood. The isolation of DCs in
       humans is accomplished through negative selection until a
       morphologically pure population is obtained. Recently, DC precursors
       purified from peripheral blood by negative selection have been observed
       to develop into functionally and morphologically mature DCs. In this
       report we identify three populations of cells in peripheral blood that
       have or can develop a dendritic morphology. The first population, when
       allowed to mature in culture, develops a dendritic morphology and gains
       the expression of HB15, a marker of DCs in blood, thymus, skin, and
       lymphoid organs. The second population expresses HB15 and has the
       phenotypic and morphologic characteristics of mature DCs. The third
       population is morphologically very similar to mature DCs but does not
       share the same T-cell-stimulatory activity and is the only population
       that is infectable with HIV. Understanding the heterogeneity of cells of
       dendritic lineage and/or morphology in the peripheral blood will aid in
       understanding their role as antigen-presenting cells in general and as
       potential participants in the immunopathogenesis of HIV disease.
 DE    Antigens, Surface/BLOOD  Biological Markers  Cell Division  Cell
       Separation/*METHODS  Cells, Cultured  CD4-Positive
       T-Lymphocytes/IMMUNOLOGY  Dendritic Cells/*CYTOLOGY/*VIROLOGY  Human
       HIV-1/*PATHOGENICITY  HLA-DR Antigens/BLOOD  Lymphocyte Culture Test,
       Mixed  Lymphocyte Transformation  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

