       Document 0002
 DOCN  M9580002
 TI    Expression of kappa opioid receptors in human and monkey lymphocytes.
 DT    9506
 AU    Chuang LF; Chuang TK; Killam KF Jr; Qiu Q; Wang XR; Lin JJ; Kung HF;
       Sheng W; Chao C; Yu L; et al; Department of Medical Pharmacology and
       Toxicology, University of; California, Davis 95616, USA.
 SO    Biochem Biophys Res Commun. 1995 Apr 26;209(3):1003-10. Unique
       Identifier : AIDSLINE MED/95251683
 AB    mRNA encoding the kappa opioid receptor gene sequence was identified and
       isolated from various human lymphocytic cells: CEM x174 (a hybrid of T
       and B origin) cells, Jurkat-T4 cells, human peripheral blood mononuclear
       cells (PBMC) and purified CD4+ cells. Analyzing the cDNA sequences of
       RNA transcripts spanning the putative second extracellular loop, which
       has reported dynorphin specificity, and the seventh transmembrane domain
       revealed a 100% and 95% homology in amino acid sequence to corresponding
       kappa opioid receptor sequences in human placenta and rat brain,
       respectively. Expression of a similar kappa opioid receptor sequence
       could be detected in normal monkey PBMC but not in monkey PBMC in which
       the CD4+/CD8+ cell ratio (or CD4+ cell number) was significantly reduced
       due to prolonged SIV infection. These findings suggest that human and
       monkey lymphocytes constitutively express kappa opioid receptor mRNA.
 DE    Amino Acid Sequence  Animal  B-Lymphocytes  Brain/METABOLISM  Cell Line
       Cells, Cultured  Comparative Study  CD4-CD8 Ratio  CD4-Positive
       T-Lymphocytes/*METABOLISM  Female  Flow Cytometry  *Gene Expression
       Human  Lymphocytes/*METABOLISM/VIROLOGY  Macaca mulatta  Male  Molecular
       Sequence Data  Organ Specificity  Placenta/METABOLISM  Polymerase Chain
       Reaction  Pregnancy  Receptors, Opioid, kappa/*BIOSYNTHESIS  RNA,
       Messenger/ANALYSIS/BIOSYNTHESIS  Sequence Homology, Amino Acid  Support,
       U.S. Gov't, P.H.S.  SIV/PHYSIOLOGY  T-Lymphocytes  Tumor Cells, Cultured
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

