       Document 0088
 DOCN  M9580088
 TI    Endogenous macrophage CSF production is associated with viral
       replication in HIV-1-infected human monocyte-derived macrophages.
 DT    9506
 AU    Gruber MF; Weih KA; Boone EJ; Smith PD; Clouse KA; Division of Cytokine
       Biology, Food and Drug Administration,; Bethesda, MD 20892, USA.
 SO    J Immunol. 1995 May 15;154(10):5528-35. Unique Identifier : AIDSLINE
       MED/95248120
 AB    Human monocyte-derived macrophages (MDM) cultured in medium containing
       macrophage (M) CSF are more susceptible to infection with HIV-1. M-CSF
       increases the frequency with which MDM become infected, the level of HIV
       mRNA expressed per infected cell, and the level of proviral DNA
       expressed per infected culture. Because of these effects of M-CSF on
       HIV-1 replication and the reported function of this factor as a survival
       and differentiation factor for human monocytes, we investigated whether
       HIV-1 could induce endogenous M-CSF production by MDM and the potential
       role of endogenous M-CSF on HIV-1 infection in these cells. MDM infected
       with HIV and maintained in the absence of exogenous M-CSF produced this
       cytokine endogenously at levels 5- to 24-fold higher than uninfected
       cells. In contrast, the proinflammatory cytokines IL-1, IL-6, and
       TNF-alpha and the growth factor granulocyte-macrophage CSF were not
       detected. The kinetics of M-CSF production following infection
       paralleled the kinetics of virus replication. Furthermore, enhanced
       production of M-CSF was dependent on viral entry and active replication
       of HIV-1. Thus, endogenous M-CSF production may contribute to the
       survival of HIV-infected MDM, enable them to function as a reservoir for
       HIV, and facilitate the spread of virus in vivo.
 DE    Blotting, Northern  Cells, Cultured  Cytokines/ANALYSIS  Human  HIV
       Infections/*IMMUNOLOGY  HIV-1/IMMUNOLOGY
       Macrophages/*IMMUNOLOGY/*VIROLOGY  Receptors, Macrophage
       Colony-Stimulating Factor/*BIOSYNTHESIS  Reverse Transcriptase/ANALYSIS
       RNA/ANALYSIS  Support, Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.
       Virus Replication/*IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

