       Document 0109
 DOCN  M9580109
 TI    High frequency of EBV association with non-random abnormalities of the
       chromosome region 1q21-25 in AIDS-related Burkitt's lymphoma-derived
       cell lines.
 DT    9506
 AU    Polito P; Cilia AM; Gloghini A; Cozzi M; Perin T; De Paoli P; Gaidano G;
       Carbone A; Division of Pathology, Istituto Nazionale di Ricovero e Cura
       a; Carattere Scientifico, Aviano, Italy.
 SO    Int J Cancer. 1995 May 4;61(3):370-4. Unique Identifier : AIDSLINE
       MED/95247332
 AB    Chromosome 1q abnormalities represent the second most frequent
       cytogenetic lesion of Burkitt lymphoma (BL) and acute lymphoblastic
       leukemia (ALL)-L3. The most frequent change is partial duplication of
       the long arm of chromosome 1, involving variable bands but consistently
       including 1q23. Among AIDS-related BL similar chromosome 1q
       abnormalities have also been found. We have now characterized in detail
       the chromosome 1q abnormalities of 4 AIDS-BL cell lines and compared
       them to other molecular features of the tumor clone, namely infection by
       Epstein Barr virus (EBV). Immunophenotypic characteristics were also
       assessed by conventional in situ immunocytochemical and flow cytometric
       procedures. The B-cell origin of all cell lines was demonstrated by the
       expression of B-cell-restricted markers (e.g., CD19). Analysis of Ig
       light chains confirmed their monoclonal nature. The t(8;14) was present
       in 3 of the 4 lines, whereas variant translocation t(8;22) was detected
       in the remaining cell line. Additional chromosomal changes were found in
       all cases, with chromosome 1 being involved in all. Structural changes
       encompassed in each case the 1q21-25 bands, in either duplication or
       partial trisomy. EBER ISH studies identified EBV association in 3 of the
       4 AIDS-BL cell lines in contrast to previous studies of BL of
       immunocompetent individuals. Our findings of a high frequency of
       chromosome 1q abnormalities in EBV-infected AIDS-related BL cell lines
       demonstrate that such chromosomal abnormality and EBV positivity are not
       mutually exclusive and are possibly independent factors, whereas their
       close association in AIDS may be related to the immunodeficiency.
 DE    Acquired Immunodeficiency Syndrome/*COMPLICATIONS/GENETICS  Burkitt's
       Lymphoma/*EPIDEMIOLOGY/*GENETICS/VIROLOGY  Cell Line  *Chromosome
       Abnormalities  Chromosome Mapping  *Chromosomes, Human, Pair 1  Genetic
       Markers  Herpesvirus 4, Human/*ISOLATION & PURIF  Human  In Situ
       Hybridization  Karyotyping  Support, Non-U.S. Gov't  Trisomy  Tumor
       Cells, Cultured  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

