       Document 0112
 DOCN  M9580112
 TI    Regulation of macrophage activation and human immunodeficiency virus
       production by invasive Salmonella strains.
 DT    9506
 AU    Mizel SB; Kucera LS; Richardson SH; Ciacci F; Iyer NP; Department of
       Microbiology and Immunology, Wake Forest University; Medical Center,
       Winston-Salem, North Carolina 27157, USA.
 SO    Infect Immun. 1995 May;63(5):1820-6. Unique Identifier : AIDSLINE
       MED/95247267
 AB    Salmonellae possess the ability to adhere to and invade macrophages and
       in so doing trigger a number of intracellular events that are associated
       with cellular activation. As an initial approach to defining the
       mechanisms by which invasive salmonellae alter macrophage function, we
       have explored the impact of Salmonella infection on the production of
       human immunodeficiency virus (HIV) in U1 cells, a promonocytic cell line
       latently infected with the virus. Infection of U1 cells with a
       pathogenic strain of Salmonella enteritidis resulted in a marked
       induction of macrophage activation and HIV production. The stimulatory
       effect of salmonellae was mediated by signals other than
       lipopolysaccharide. Salmonella mutants with specific defects in invasion
       or intracellular survival were markedly less effective in the induction
       of HIV production. In contrast to S. enteritidis, strains of Yersinia
       enterocolitica, Legionella pneumophila, and Escherichia coli did not
       induce HIV production. However, all of these bacteria induced comparable
       levels of gene expression mediated by the HIV long terminal repeat. The
       results of this study are consistent with the notion that invasive
       salmonellae possess the ability to activate the macrophage by at least
       one mechanism that is not shared with several other species of
       gram-negative bacteria. Furthermore, the expression of this unique
       property is maximal with Salmonella strains that are not only invasive
       but also capable of prolonged survival within the macrophage. Our
       results indicate that the U1 cell line may be a very useful model system
       with which to examine the biochemical pathways by which internalized
       salmonellae modulate the activation state of the macrophage.
 DE    Comparative Study  Escherichia coli/IMMUNOLOGY  Gene Expression
       Regulation, Viral  HIV/*GROWTH & DEVELOPMENT/GENETICS  HIV Long Terminal
       Repeat/GENETICS  Legionella pneumophila/IMMUNOLOGY
       Lipopolysaccharides/PHARMACOLOGY  *Macrophage Activation
       Macrophages/MICROBIOLOGY/VIROLOGY  Salmonella/*IMMUNOLOGY/PATHOGENICITY
       Support, U.S. Gov't, P.H.S.  Tumor Cells, Cultured  *Virus Latency
       Yersinia enterocolitica/IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

