       Document 0144
 DOCN  M9580144
 TI    The antitumor effect of tumor-draining lymph node cells activated by
       both anti-CD3 monoclonal antibody and activated B cells as
       costimulatory-signal-providing cells.
 DT    9506
 AU    Okamoto T; Harada M; Shinomiya Y; Matsuzaki G; Nomoto K; Department of
       Immunology, Kyushu University, Fukuoka, Japan.
 SO    Cancer Immunol Immunother. 1995 Mar;40(3):173-81. Unique Identifier :
       AIDSLINE MED/95246049
 AB    To establish an efficient cell-culture system for adoptive
       immunotherapy, we attempted to use lipopolysaccharide(LPS)-activated B
       cells (LPS blasts) as costimulatory-signal-providing cells in the in
       vitro induction of antitumor effector cells. Both normal and
       tumor-draining lymph node cells were efficiently activated by both
       anti-CD3 monoclonal antibody (mAb) and LPS blasts, and subsequently
       expanded by a low dose of interleukin-2 (IL-2; anti-CD3 mAb and LPS
       blasts/IL-2). The expanded cells were predominantly CD8+ T cells and
       showed a low level of tumor-specific cytotoxic T lymphocyte (CTL)
       activity. The adoptive transfer of B16-melanoma-draining lymph node
       cells expanded by anti-CD3 mAb and LPS blasts/IL-2 showed significant
       antitumor effect against the established metastases of B16 in
       combination with intraperitoneal injections of IL-2. This treatment
       cured all B16-bearing mice. In addition, these mice also showed
       tumor-specific protective immunity against B16 at the rechallenge.
       Considering that activated B cells express several kinds of
       costimulatory molecules, these findings thus indicate an efficacy of
       costimulation that is derived from activated B cells for the in vitro
       induction of tumor-specific CTL, in co-operation with anti-CD3 mAb. The
       culture system presented here may thus be therapeutically useful,
       providing potent effectors for adoptive immunotherapy against various
       types of cancer.
 DE    Animal  Antibodies, Monoclonal/*IMMUNOLOGY  B-Lymphocyte
       Subsets/*IMMUNOLOGY  Cytotoxicity Tests, Immunologic  Cytotoxicity,
       Immunologic/*IMMUNOLOGY  CD8-Positive T-Lymphocytes/IMMUNOLOGY  Female
       Immunotherapy, Adoptive  Lipopolysaccharides/IMMUNOLOGY  Lymph
       Nodes/*IMMUNOLOGY/PATHOLOGY  Melanoma, Experimental/*IMMUNOLOGY/THERAPY
       Mice  Mice, Inbred C57BL  Receptors, IgG/ANALYSIS/IMMUNOLOGY  Support,
       Non-U.S. Gov't  T-Lymphocytes, Cytotoxic/IMMUNOLOGY  Tumor Cells,
       Cultured/IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

