       Document 0737
 DOCN  M9590737
 TI    Expression of the human mucosal lymphocyte antigen, HML-1, by T cells
       activated with mitogen or specific antigen in vitro.
 DT    9509
 AU    Brew R; West DC; Burthem J; Christmas SE; Department of Immunology,
       Royal Liverpool University Hospital,; UK.
 SO    Scand J Immunol. 1995 Jun;41(6):553-62. Unique Identifier : AIDSLINE
       MED/95288585
 AB    Expression of the human mucosal lymphocyte antigen, HML-1 (CD103),
       recently identified as a novel alpha E beta 7 integrin, was studied on
       peripheral blood lymphocytes activated with mitogen or specific antigen.
       HML-1 was up-regulated on PHA activated T-lymphoblasts cultured in
       100IU/ml interleukin-2 (IL-2), reaching a peak of > 50% positive cells
       at day 7, and expression was maintained at this level throughout the
       28-day culture period. Following a transient decrease in the percentage
       of L-selectin cells, expression of this molecule was maintained on most
       PHA T-lymphoblasts. Cells activated by purified protein derivative of M.
       tuberculosis (PPD) or in mixed lymphocyte culture also up-regulated and
       maintained HML-1 expression for 14 days. In contrast, in all cases the
       percentage of CD25+ cells rose initially but subsequently declined over
       the same time periods. When freshly isolated cells from tonsil, spleen,
       mesenteric lymph node and lung were analysed, only lung contained
       significant numbers (39 +/- 6%) of HML-1+ cells. In both freshly
       isolated and activated cell populations the great majority of HML-1+
       cells co-expressed CD8 although some HML-1+ CD8- cells were also
       present. Production of TGF-beta 1 peaked early during T-lymphoblast and
       MLR cultures and was not related to induction of HML-1 expression.
       Immunoprecipitation studies showed that the HML-1 molecule expressed on
       10-day PHA T-lymphoblasts was indistinguishable from that found on
       intestinal intraepithelial lymphocytes and that no alpha 4 beta 7
       integrin was expressed by these cells. Although HML-1 expression is
       essentially restricted to mucosal leucocytes in vivo, these experiments
       show that it is readily induced and maintained along with co-expression
       of L-selectin following CD8+ T-lymphocyte activation in vitro.
 DE    Antibodies, Monoclonal/IMMUNOLOGY  Antigens/IMMUNOLOGY  Antigens,
       CD/*BIOSYNTHESIS  Cells, Cultured  CD8-Positive
       T-Lymphocytes/*IMMUNOLOGY  Flow Cytometry  Human  Intestinal
       Mucosa/IMMUNOLOGY  Lung/CYTOLOGY  Lymph Nodes/CYTOLOGY  Lymphocyte
       Transformation/*IMMUNOLOGY  Lymphoid Tissue/CYTOLOGY
       Mitogens/IMMUNOLOGY  Precipitin Tests  Receptors,
       Interleukin-2/BIOSYNTHESIS  Spleen/CYTOLOGY  Support, Non-U.S. Gov't
       T-Lymphocyte Subsets/*IMMUNOLOGY  Tonsil/CYTOLOGY  Transforming Growth
       Factor beta/BIOSYNTHESIS  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

