       Document 0775
 DOCN  M9590775
 TI    Aberrant Gag protein composition of a human immunodeficiency virus type
       1 vif mutant produced in primary lymphocytes.
 DT    9509
 AU    Simm M; Shahabuddin M; Chao W; Allan JS; Volsky DJ; Molecular Virology
       Laboratory, St. Luke's-Roosevelt Hospital; Center and College of
       Physicians & Surgeons, Columbia University,; New York, New York 10019,
       USA.
 SO    J Virol. 1995 Jul;69(7):4582-6. Unique Identifier : AIDSLINE
       MED/95287525
 AB    Productive, spreading infection of peripheral blood lymphocytes (PBL)
       with human immunodeficiency virus type 1 (HIV-1) requires the viral
       protein Vif. To study the requirement for vif in this system, we
       infected PBL with a phenotypically complemented HIV-1 clone mutated in
       vif. Progeny virus was produced which was noninfectious in PBL but
       replicated in SupT1 cells. Analysis of metabolically labeled proteins of
       sedimentable extracellular particles made in PBL by
       radioimmunoprecipitation with either serum from a patient with AIDS or a
       monoclonal antibody reactive with HIV-1 Gag proteins revealed that
       vif-negative but not wild-type particles carry higher levels of p55,
       p41, and p38 Gag-specific proteins compared with those of p24. Similar
       results were obtained with sucrose-purified virions. Our data indicate
       that vif plays a role in Gag protein processing or in incorporation of
       processed Gag products into mature virions. The presence of unprocessed
       precursor Gag polyprotein (Pr55gag) and other Gag processing
       intermediates in PBL-derived vif-negative extracellular particles may
       contribute to the reduced infectivity of this virus.
 DE    Gene Products, gag/*ANALYSIS  Gene Products, vif/*PHYSIOLOGY  Human
       HIV-1/*CHEMISTRY/PHYSIOLOGY  Lymphocytes/*VIROLOGY  Mutation  Protein
       Precursors/ANALYSIS  Support, Non-U.S. Gov't  Support, U.S. Gov't,
       P.H.S.  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

